Tenascin-X expression in tumor cells and fibroblasts: Glucocorticoids as negative regulators in fibroblasts

Takao Sakai, Yusuke Furukawa, Ruth Chiquet-Ehrismann, Mitsuru Nakamura, Seiichi Kitagawa, Toshimichi Ikemura, Ken Ichi Matsumoto

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Tenascin-X has recently been shown to be a novel member of the tenascin family and its distribution is often reciprocal to that of tenascin-C in the developing mouse embryo. We have investigated the expression of tenascin-X in fibroblasts and carcinoma cells in culture. Tenascin-X protein was secreted in vitro in the conditioned media at an apparent molecular mass of ~ 450 kDa. In addition fibroblasts contained a major tenascin-X isoform of 220 kDa. On northern blots, a single major transcript with a size of ~ 13 kb was detected. No overexpression of tenascin-X protein was found in primary fibroblasts of the tenascin-C-gene knockout mice. Steroid hormone glucocorticoids, were found to downregulate tenascin-X mRNA levels and protein synthesis in fibroblasts but not carcinoma cells at physiological concentrations. None of the growth factors or cytokines examined affected the expression level of tenascin-X. As in vivo study, carcinoma cells were transplanted into nude mice. In contrast to the ubiquitous presence of tenascin-X in adult skin, expression of tenascin-X protein during tumorigenesis was found to be downregulated considerably not only in tumor cells themselves but also in tumor stroma. These findings provide evidence that the expression of tenascin-X can be influenced by stromal-epithelial interactions. We have identified glucocorticoids as physiological inhibitors of tenascin-X and suggest that glucocorticoids may in part participate in the downregulation of tenascin-X in fibroblasts in vivo.

Original languageEnglish
Pages (from-to)2069-2077
Number of pages9
JournalJournal of cell science
Volume109
Issue number8
Publication statusPublished - 08-1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology

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