Testosterone up-regulates aquaporin-4 expression in cultured astrocytes

Feng Gu, Ryuji Hata, Kazuko Toku, Lihua Yang, Yong Jie Ma, Nobuji Maeda, Masahiro Sakanaka, Junya Tanaka

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)


Aquaporin-4 (AQP4) is located on astrocyte endfeet that face blood vessels in the brain and in the pia. It is thought to play a crucial role in the development of brain edema. To confirm the notion that sex steroids and dexamethasone influence brain edema through AQP4 regulation, we investigated the effects of 17β-estradiol, testosterone, and dexamethasone on the expression of AQP4 in cultured astrocytes. Testosterone significantly up-regulated AQP4 at the level of both protein and mRNA. At a concentration of 100 nM, testosterone significantly increased AQP4 protein levels and ameliorated the osmotic fragility of astrocytes from hypoosmotic stress, suggesting that the increased levels of AQP4 facilitated the testosterone function. Moreover, this effect was attenuated by the protein kinase C activator 12-Otetradecanoylphorbol 13-acetate, which can rapidly decrease AQP4 mRNA expression, indicating that the response was specific. These results indicate that AQP4 can alter the osmotic fragility of astrocytes and that testosterone can influence brain edema through AQP4 regulation, whereas 17β-estradiol and dexamethasone cannot.

Original languageEnglish
Pages (from-to)709-715
Number of pages7
JournalJournal of Neuroscience Research
Issue number6
Publication statusPublished - 15-06-2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience


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