The 5′-triphosphates of 3′-azido-3′-deoxythymidine and 2′, 3′-dideoxynucleosides inhibit DNA polymerase γ by different mechanisms

Shunji Izuta, Mineo Saneyoshi, Takeshi Sakurai, Motoshi Suzuki, Kiyohide Kojima, Shonen Yoshida

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Inhibition mechanisms of 5′-triphosphates of 3′-azido-3′-deoxythymidine (AZT-TP) and 3′-deoxythymidine (ddTTP) on extensively purified DNA polymerase γ from bovine testes were examined by analysis of the products synthesized on singly primed M13 single-stranded DNA or synthetic oligonucleotide template-primer in the presence of analogues. The results indicate that AZT-TP inhibits DNA polymerase γ in competition with dTTP but is not incorporated into DNA, whereas ddTTP is incorporated into DNA and causes chain termination. In contrast, both analogues were used by reverse transcriptase and caused chain termination.

Original languageEnglish
Pages (from-to)776-783
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume179
Issue number2
DOIs
Publication statusPublished - 16-09-1991

Fingerprint

Dideoxynucleosides
Zidovudine
DNA-Directed DNA Polymerase
splenotritin
DNA Primers
Single-Stranded DNA
RNA-Directed DNA Polymerase
DNA
Oligonucleotides
Thymidine
Testis
3'-azido-3'-deoxythymidine 5'-triphosphate
triphosphoric acid
2',3'-dideoxythymidine triphosphate

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "Inhibition mechanisms of 5′-triphosphates of 3′-azido-3′-deoxythymidine (AZT-TP) and 3′-deoxythymidine (ddTTP) on extensively purified DNA polymerase γ from bovine testes were examined by analysis of the products synthesized on singly primed M13 single-stranded DNA or synthetic oligonucleotide template-primer in the presence of analogues. The results indicate that AZT-TP inhibits DNA polymerase γ in competition with dTTP but is not incorporated into DNA, whereas ddTTP is incorporated into DNA and causes chain termination. In contrast, both analogues were used by reverse transcriptase and caused chain termination.",
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The 5′-triphosphates of 3′-azido-3′-deoxythymidine and 2′, 3′-dideoxynucleosides inhibit DNA polymerase γ by different mechanisms. / Izuta, Shunji; Saneyoshi, Mineo; Sakurai, Takeshi; Suzuki, Motoshi; Kojima, Kiyohide; Yoshida, Shonen.

In: Biochemical and Biophysical Research Communications, Vol. 179, No. 2, 16.09.1991, p. 776-783.

Research output: Contribution to journalArticle

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