TY - JOUR
T1 - The APC/C activator Cdh1 regulates the G2/M transition during differentiation of placental trophoblast stem cells
AU - Naoe, Hideaki
AU - Chiyoda, Tatsuyuki
AU - Ishizawa, Jo
AU - Masuda, Kenta
AU - Saya, Hideyuki
AU - Kuninaka, Shinji
N1 - Funding Information:
We thank Drs. G. Leone and P. L. Wenzel for providing in situ hybridization probes; N. Tomotsugu for assistance in the statistical analyses; I. Ishimatsu for technical assistance in the histological analysis; T. Shinoda for technical assistance in the analysis of nuclear size; N. Suzuki for animal maintenance; and K. Arai for secretarial assistance. This study was supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan (to S.K. ( 20058033 ) and H.S. ( 17013070 )).
PY - 2013/1/11
Y1 - 2013/1/11
N2 - Differentiation of placental trophoblast stem (TS) cells to trophoblast giant (TG) cells is accompanied by transition from a mitotic cell cycle to an endocycle. Here, we report that Cdh1, a regulator of the anaphase-promoting complex/cyclosome (APC/C), negatively regulates mitotic entry upon the mitotic/endocycle transition. TS cells derived from homozygous Cdh1 gene-trapped (Cdh1GT/GT) murine embryos accumulated mitotic cyclins and precociously entered mitosis after induction of TS cell differentiation, indicating that Cdh1 is required for the switch from mitosis to the endocycle. Furthermore, the Cdh1GT/GT TS cells and placenta showed aberrant expression of placental differentiation markers. These data highlight an important role of Cdh1 in the G2/M transition during placental differentiation.
AB - Differentiation of placental trophoblast stem (TS) cells to trophoblast giant (TG) cells is accompanied by transition from a mitotic cell cycle to an endocycle. Here, we report that Cdh1, a regulator of the anaphase-promoting complex/cyclosome (APC/C), negatively regulates mitotic entry upon the mitotic/endocycle transition. TS cells derived from homozygous Cdh1 gene-trapped (Cdh1GT/GT) murine embryos accumulated mitotic cyclins and precociously entered mitosis after induction of TS cell differentiation, indicating that Cdh1 is required for the switch from mitosis to the endocycle. Furthermore, the Cdh1GT/GT TS cells and placenta showed aberrant expression of placental differentiation markers. These data highlight an important role of Cdh1 in the G2/M transition during placental differentiation.
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U2 - 10.1016/j.bbrc.2012.11.075
DO - 10.1016/j.bbrc.2012.11.075
M3 - Article
C2 - 23206702
AN - SCOPUS:84872334418
SN - 0006-291X
VL - 430
SP - 757
EP - 762
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -