The Aurora-B-mediated phosphorylation of SHCBP1 regulates cytokinetic furrow ingression

Eri Asano, Hitoki Hasegawa, Toshinori Hyodo, Satoko Ito, Masao Maeda, Masahide Takahashi, Michinari Hamaguchi, Takeshi Senga

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Centralspindlin, which is composed of MgcRacGAP and MKLP1, is essential for central spindle formation and cytokinetic furrow ingression. MgcRacGAP utilizes its GAP domain to inactivate Rac1 and induce furrow ingression in mammalian cells. In this report, we present a novel regulatory mechanism for furrowing that is mediated by the phosphorylation of SHC SH2-domain binding protein 1 (SHCBP1), a binding partner of centralspindlin, by Aurora B (AurB). AurB phosphorylates Ser634 of SHCBP1 during mitosis. We generated a phosphorylation site mutant, S634A-SHCBP1, which was prematurely recruited to the central spindle during anaphase and inhibited furrowing. An in vitro GAP assay demonstrated that SHCBP1 can suppress the MgcRacGAP-mediated inactivation of Rac1. In addition, the inhibition of Rac1 activity rescued the furrowing defect induced by S634A-SHCBP1 expression. Thus, AurB phosphorylates SHCBP1 to prevent the premature localization of SHCBP1 to the central spindle and ensures that MgcRacGAP inactivates Rac1 to promote the ingression of the cytokinetic furrow.

Original languageEnglish
Pages (from-to)3263-3270
Number of pages8
JournalJournal of Cell Science
Volume126
Issue number15
DOIs
Publication statusPublished - 09-09-2013
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology

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