The biological activity of ABA-1-like protein from Ascaris lumbricoides

Riho Muto, Shinjiro Imai, Hiroyuki Tezuka, Yuko Furuhashi, Koichiro Fujita

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The elevation of non-specific IgE (totai IgE) in Ascaris infection can be seen one week after infection, and reaches a peak after approximately two weeks. It has been reported that ABA-1 protein is the main constituent in the pseudocoelomic fluid of Ascaris suum. To investigate the effect of the ABA-1-like protein from Ascaris lumbricoides (ALB), the cDNA was cloned by reverse transcriptase polymerase chain reaction, using original primers based on the consensus sequences of ABA-1 and TBA-1, that is an ABA-1-like protein from Toxocara canis. The clone was sequenced, we constructed the recombinant polyprotein of ALB (rALB14 and rALB7) based on the ALB sequence, and rALB was administrated to BALB/c mice. Fourteen days after inoculation with rALB14 which is the full length of ALB, the elevation of total IgE which we supposed to contain non-specific IgE was observed, and the results were as we expected. Furthermore, in an in-vitro experiment, we confirmed that the spleen cells proliferated when stimulated by rALB14 and concanavalin A. Therefore, the whole conformation of ALB is considered to be involved in the elevation of non-specific IgE, and is involved in the activation of T cells.

Original languageEnglish
Pages (from-to)95-104
Number of pages10
JournalJournal of Medical and Dental Sciences
Volume48
Issue number3
Publication statusPublished - 01-12-2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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