The BOLF1 gene is necessary for effective Epstein–Barr viral infectivity

H. M.Abdullah Al Masud, Takahiro Watanabe, Yoshitaka Sato, Fumi Goshima, Hiroshi Kimura, Takayuki Murata

Research output: Contribution to journalArticle

Abstract

The Epstein–Barr virus (EBV) is a causative agent of infectious mononucleosis and several malignancies. Here, we focused on an EBV lytic protein, BOLF1, which is conserved throughout the herpesvirus family and is reported to be a virion tegument protein. We first constructed BOLF1-deficient viruses using the bacterial artificial chromosome (BAC) and CRISPR/Cas9 systems. Although the loss of BOLF1 had almost no effect on viral protein expression, DNA synthesis, or extracellular progeny release, EBV infectivity was significantly reduced. Further analysis showed that nuclear transportation of the incoming virus was decreased by the disruption of BOLF1. Our results indicate that BOLF1enhances the infectious potential of progeny virions, at least partly by increasing nuclear transportation of incoming nucleocapsids. We also found that BOLF1 interacted with BKRF4, and the BOLF1 and BKRF4 proteins were localized in the nucleus and perinuclear area, during the viral lytic cycle.

Original languageEnglish
Pages (from-to)114-125
Number of pages12
JournalVirology
Volume531
DOIs
Publication statusPublished - 01-05-2019

Fingerprint

Viruses
Virion
Genes
Clustered Regularly Interspaced Short Palindromic Repeats
Virus Release
Bacterial Artificial Chromosomes
Nucleocapsid
Infectious Mononucleosis
Proteins
Herpesviridae
Viral Proteins
DNA
Neoplasms

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Masud, H. M. A. A., Watanabe, T., Sato, Y., Goshima, F., Kimura, H., & Murata, T. (2019). The BOLF1 gene is necessary for effective Epstein–Barr viral infectivity. Virology, 531, 114-125. https://doi.org/10.1016/j.virol.2019.02.015
Masud, H. M.Abdullah Al ; Watanabe, Takahiro ; Sato, Yoshitaka ; Goshima, Fumi ; Kimura, Hiroshi ; Murata, Takayuki. / The BOLF1 gene is necessary for effective Epstein–Barr viral infectivity. In: Virology. 2019 ; Vol. 531. pp. 114-125.
@article{3a6c09df8e0e4ab98f84e7d07190f671,
title = "The BOLF1 gene is necessary for effective Epstein–Barr viral infectivity",
abstract = "The Epstein–Barr virus (EBV) is a causative agent of infectious mononucleosis and several malignancies. Here, we focused on an EBV lytic protein, BOLF1, which is conserved throughout the herpesvirus family and is reported to be a virion tegument protein. We first constructed BOLF1-deficient viruses using the bacterial artificial chromosome (BAC) and CRISPR/Cas9 systems. Although the loss of BOLF1 had almost no effect on viral protein expression, DNA synthesis, or extracellular progeny release, EBV infectivity was significantly reduced. Further analysis showed that nuclear transportation of the incoming virus was decreased by the disruption of BOLF1. Our results indicate that BOLF1enhances the infectious potential of progeny virions, at least partly by increasing nuclear transportation of incoming nucleocapsids. We also found that BOLF1 interacted with BKRF4, and the BOLF1 and BKRF4 proteins were localized in the nucleus and perinuclear area, during the viral lytic cycle.",
author = "Masud, {H. M.Abdullah Al} and Takahiro Watanabe and Yoshitaka Sato and Fumi Goshima and Hiroshi Kimura and Takayuki Murata",
year = "2019",
month = "5",
day = "1",
doi = "10.1016/j.virol.2019.02.015",
language = "English",
volume = "531",
pages = "114--125",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",

}

Masud, HMAA, Watanabe, T, Sato, Y, Goshima, F, Kimura, H & Murata, T 2019, 'The BOLF1 gene is necessary for effective Epstein–Barr viral infectivity', Virology, vol. 531, pp. 114-125. https://doi.org/10.1016/j.virol.2019.02.015

The BOLF1 gene is necessary for effective Epstein–Barr viral infectivity. / Masud, H. M.Abdullah Al; Watanabe, Takahiro; Sato, Yoshitaka; Goshima, Fumi; Kimura, Hiroshi; Murata, Takayuki.

In: Virology, Vol. 531, 01.05.2019, p. 114-125.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The BOLF1 gene is necessary for effective Epstein–Barr viral infectivity

AU - Masud, H. M.Abdullah Al

AU - Watanabe, Takahiro

AU - Sato, Yoshitaka

AU - Goshima, Fumi

AU - Kimura, Hiroshi

AU - Murata, Takayuki

PY - 2019/5/1

Y1 - 2019/5/1

N2 - The Epstein–Barr virus (EBV) is a causative agent of infectious mononucleosis and several malignancies. Here, we focused on an EBV lytic protein, BOLF1, which is conserved throughout the herpesvirus family and is reported to be a virion tegument protein. We first constructed BOLF1-deficient viruses using the bacterial artificial chromosome (BAC) and CRISPR/Cas9 systems. Although the loss of BOLF1 had almost no effect on viral protein expression, DNA synthesis, or extracellular progeny release, EBV infectivity was significantly reduced. Further analysis showed that nuclear transportation of the incoming virus was decreased by the disruption of BOLF1. Our results indicate that BOLF1enhances the infectious potential of progeny virions, at least partly by increasing nuclear transportation of incoming nucleocapsids. We also found that BOLF1 interacted with BKRF4, and the BOLF1 and BKRF4 proteins were localized in the nucleus and perinuclear area, during the viral lytic cycle.

AB - The Epstein–Barr virus (EBV) is a causative agent of infectious mononucleosis and several malignancies. Here, we focused on an EBV lytic protein, BOLF1, which is conserved throughout the herpesvirus family and is reported to be a virion tegument protein. We first constructed BOLF1-deficient viruses using the bacterial artificial chromosome (BAC) and CRISPR/Cas9 systems. Although the loss of BOLF1 had almost no effect on viral protein expression, DNA synthesis, or extracellular progeny release, EBV infectivity was significantly reduced. Further analysis showed that nuclear transportation of the incoming virus was decreased by the disruption of BOLF1. Our results indicate that BOLF1enhances the infectious potential of progeny virions, at least partly by increasing nuclear transportation of incoming nucleocapsids. We also found that BOLF1 interacted with BKRF4, and the BOLF1 and BKRF4 proteins were localized in the nucleus and perinuclear area, during the viral lytic cycle.

UR - http://www.scopus.com/inward/record.url?scp=85063112713&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063112713&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2019.02.015

DO - 10.1016/j.virol.2019.02.015

M3 - Article

C2 - 30856483

AN - SCOPUS:85063112713

VL - 531

SP - 114

EP - 125

JO - Virology

JF - Virology

SN - 0042-6822

ER -