The C-terminal domain of the adenomatous polyposis coli (Apc) protein is involved in thyroid morphogenesis and function

Atsushi Yokoyama, Ryuji Nomura, Masafumi Kurosumi, Atsushi Shimomura, Takanori Onouchi, Akiko Iizuka-Kogo, Ron Smits, Naohisa Oda, Riccardo Fodde, Mitsuyasu Itoh, Takao Senda

Research output: Contribution to journalArticle

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Abstract

Adenomatous polyposis coli (APC) is a multifunctional protein as well as a tumor suppressor. To determine the functions of the C-terminal domain of Apc, we have investigated Apc 1638T/1638T mice, which express a truncated Apc that lacks the C-terminal domain. Apc 1638T/1638T mice are tumor free and exhibit growth retardation. In the present study, we analyzed the morphology and functions of the thyroid gland in Apc 1638T/1638T mice. There was no significant difference in the basal concentration of serum thyroid hormones between Apc 1638T/1638T and Apc +/+ mice. Thyroid follicle size was significantly larger in Apc 1638T/1638T mice than in Apc +/+ mice. The extent of serum T4 elevation following exogenous thyroid-stimulating hormone (TSH) injection was lower in Apc 1638T/1638T mice than in Apc +/+ mice. TSH also induced a greater reduction in thyroid follicle size in Apc 1638T/1638T mice than in Apc +/+ mice. Analyses using immunohistochemistry and electron microscopy indicated that follicular epithelial cells in Apc 1638T/1638T mice had an enlarged rough endoplasmic reticulum of irregular shape. These results suggest that the C-terminal domain of Apc is involved in thyroid morphology and function.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalMedical Molecular Morphology
Volume44
Issue number4
DOIs
Publication statusPublished - 01-12-2011

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Adenomatous Polyposis Coli Protein
Adenomatous Polyposis Coli
Morphogenesis
Thyroid Gland
Thyrotropin

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology

Cite this

Yokoyama, Atsushi ; Nomura, Ryuji ; Kurosumi, Masafumi ; Shimomura, Atsushi ; Onouchi, Takanori ; Iizuka-Kogo, Akiko ; Smits, Ron ; Oda, Naohisa ; Fodde, Riccardo ; Itoh, Mitsuyasu ; Senda, Takao. / The C-terminal domain of the adenomatous polyposis coli (Apc) protein is involved in thyroid morphogenesis and function. In: Medical Molecular Morphology. 2011 ; Vol. 44, No. 4. pp. 207-212.
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abstract = "Adenomatous polyposis coli (APC) is a multifunctional protein as well as a tumor suppressor. To determine the functions of the C-terminal domain of Apc, we have investigated Apc 1638T/1638T mice, which express a truncated Apc that lacks the C-terminal domain. Apc 1638T/1638T mice are tumor free and exhibit growth retardation. In the present study, we analyzed the morphology and functions of the thyroid gland in Apc 1638T/1638T mice. There was no significant difference in the basal concentration of serum thyroid hormones between Apc 1638T/1638T and Apc +/+ mice. Thyroid follicle size was significantly larger in Apc 1638T/1638T mice than in Apc +/+ mice. The extent of serum T4 elevation following exogenous thyroid-stimulating hormone (TSH) injection was lower in Apc 1638T/1638T mice than in Apc +/+ mice. TSH also induced a greater reduction in thyroid follicle size in Apc 1638T/1638T mice than in Apc +/+ mice. Analyses using immunohistochemistry and electron microscopy indicated that follicular epithelial cells in Apc 1638T/1638T mice had an enlarged rough endoplasmic reticulum of irregular shape. These results suggest that the C-terminal domain of Apc is involved in thyroid morphology and function.",
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Yokoyama, A, Nomura, R, Kurosumi, M, Shimomura, A, Onouchi, T, Iizuka-Kogo, A, Smits, R, Oda, N, Fodde, R, Itoh, M & Senda, T 2011, 'The C-terminal domain of the adenomatous polyposis coli (Apc) protein is involved in thyroid morphogenesis and function', Medical Molecular Morphology, vol. 44, no. 4, pp. 207-212. https://doi.org/10.1007/s00795-010-0529-9

The C-terminal domain of the adenomatous polyposis coli (Apc) protein is involved in thyroid morphogenesis and function. / Yokoyama, Atsushi; Nomura, Ryuji; Kurosumi, Masafumi; Shimomura, Atsushi; Onouchi, Takanori; Iizuka-Kogo, Akiko; Smits, Ron; Oda, Naohisa; Fodde, Riccardo; Itoh, Mitsuyasu; Senda, Takao.

In: Medical Molecular Morphology, Vol. 44, No. 4, 01.12.2011, p. 207-212.

Research output: Contribution to journalArticle

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AU - Yokoyama, Atsushi

AU - Nomura, Ryuji

AU - Kurosumi, Masafumi

AU - Shimomura, Atsushi

AU - Onouchi, Takanori

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AU - Smits, Ron

AU - Oda, Naohisa

AU - Fodde, Riccardo

AU - Itoh, Mitsuyasu

AU - Senda, Takao

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