TY - JOUR
T1 - The cell surface-expressed HSC70-like molecule preferentially reacts with the rat T-cell receptor Vδ6 family
AU - Kishi, A.
AU - Ichinohe, T.
AU - Hirai, I.
AU - Kamiguchi, K.
AU - Tamura, Y.
AU - Kinebuchi, M.
AU - Torigoe, T.
AU - Ichimiya, S.
AU - Kondo, N.
AU - Ishitani, K.
AU - Yoshikawa, T.
AU - Kondo, M.
AU - Matsuura, A.
AU - Sato, N.
N1 - Funding Information:
Acknowledgements This work is dedicated to Dr. Masahiko Ohta, who died of gastric cancer in 1996 at the age of 33. He was one of the main investigators in the research into disease-specific T lymphocytes. This work was supported by the Uehara Foundation, the Akiyama Foundation, and a Grant-in Aid for Scientific Research from the Ministry of Education, Culture and Science of Japan.
PY - 2001
Y1 - 2001
N2 - We previously showed that the cell surface-expressed Mr 70,000 heat shock cognate (hsc70, a constitutively expressed member of the hsp70 family) protein-like molecule (#067 molecule) interacts with rat CD3+, CD4-, CD8-, T-cell receptor (TCR)αβ-, natural killer recetor-P1- T cells. This 70hsc-like molecule was also suggested to present cellular peptide antigens to these T cells. In the present study, we identified the genetic structure of the TCR by establishing T-cell hybridomas between these T cells and mouse BW5147 cells. Our data indicated that these T cells preferentially used TCRs with the Vδ6 family. Analysis of the nucleotide sequence of the CDR3 junctional portion showed that there are substantial diversities, with insertion of seven to nine amino acid residues. These data provide indirect evidences for our hypothesis that an hsc70-like molecule could be presented together with cellular peptide antigens to particular T cells with TCR γδ chains. Since the expression of this hsc70-like #067 antigen on the cell surface is usually induced along with cell transformation by activated oncogenes, T cells with the TCR Vδ6 family are likely to contribute to host resistance to tumor cells.
AB - We previously showed that the cell surface-expressed Mr 70,000 heat shock cognate (hsc70, a constitutively expressed member of the hsp70 family) protein-like molecule (#067 molecule) interacts with rat CD3+, CD4-, CD8-, T-cell receptor (TCR)αβ-, natural killer recetor-P1- T cells. This 70hsc-like molecule was also suggested to present cellular peptide antigens to these T cells. In the present study, we identified the genetic structure of the TCR by establishing T-cell hybridomas between these T cells and mouse BW5147 cells. Our data indicated that these T cells preferentially used TCRs with the Vδ6 family. Analysis of the nucleotide sequence of the CDR3 junctional portion showed that there are substantial diversities, with insertion of seven to nine amino acid residues. These data provide indirect evidences for our hypothesis that an hsc70-like molecule could be presented together with cellular peptide antigens to particular T cells with TCR γδ chains. Since the expression of this hsc70-like #067 antigen on the cell surface is usually induced along with cell transformation by activated oncogenes, T cells with the TCR Vδ6 family are likely to contribute to host resistance to tumor cells.
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U2 - 10.1007/s002510100335
DO - 10.1007/s002510100335
M3 - Article
C2 - 11486277
AN - SCOPUS:0034886942
SN - 0093-7711
VL - 53
SP - 401
EP - 409
JO - Immunogenetics
JF - Immunogenetics
IS - 5
ER -