The clinical features of patients with a Y93H variant of hepatitis C virus detected by a PCR invader assay

Toshiki Kan, Senju Hashimoto, Naoto Kawabe, Michihito Murao, Takuji Nakano, Hiroaki Shimazaki, Kazunori Nakaoka, Masashi Ohki, Yuka Takagawa, Takamitsu Kurashita, Tomoki Takamura, Kentaro Yoshioka

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Resistance-associated variants (RAVs) reduce the efficacy of interferon (IFN)-free therapy with asunaprevir and daclatasvir for patients infected with hepatitis C virus (HCV) genotype 1b. The characteristics of patients with an L31 or a Y93 variant in the nonstructural 5A region detected by a polymerase chain reaction invader assay were investigated. Methods: In total, 201 patients with HCV genotype 1b were examined for L31F/M/V variants or a Y93H variant by the polymerase chain reaction invader assay. Results: L31M and Y93H variants were detected in 4.6 and 21.4 % of patients, respectively. Patients with an L31M variant had no significant characteristics. Patients with a Y93H variant had significantly higher HCV RNA levels (6.5 ± 0.5 log copies per milliliter vs 6.1 ± 0.7 log copies per milliliter, p = 0.0002), higher frequency of mutant type of the IFN-sensitivity-determining region (88.4 % vs 71.7 %, p = 0.0251), and higher frequency of TT genotype at rs8099917 of IL28B (91.7 % vs 54.3 %, p < 0.0001) than those with Y93 wild-type strains. Multivariate analysis identified HCV RNA levels [odds ratio (OR) 3.72, 95 % confidence interval (CI) 1.71–8.06, p = 0.0009] and TT genotype at rs8099917 (OR 7.45, 95 % CI 2.11–26.4, p = 0.0018) as factors associated with the presence of a Y93H variant. Conclusion: The presence of a Y93H variant was associated with higher HCV RNA levels and TT genotype at rs8099917 of IL28B. Thus, patients with a Y93H variant may be ideal candidates for IFN-based therapy rather than IFN-free therapy, although the high viral load of these patients may reduce the response rate of IFN-based therapy.

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalJournal of Gastroenterology
Volume51
Issue number1
DOIs
Publication statusPublished - 01-01-2016

All Science Journal Classification (ASJC) codes

  • Gastroenterology

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