TY - JOUR
T1 - The co-application effects of fullerene and ascorbic acid on UV-B irradiated mouse skin
AU - Ito, Shinobu
AU - Itoga, Kazuyoshi
AU - Yamato, Masayuki
AU - Akamatsu, Hirohiko
AU - Okano, Teruo
PY - 2010/1/12
Y1 - 2010/1/12
N2 - The role of fullerene as a pro-oxidant or anti-oxidant in Ultraviolet B ray (UV-B)-induced disorders in mouse skin was investigated. Fullerene gave no photo-toxic effect to UV-B-irradiated mouse skin. Since erythema was concentrated at the pore circumference in a UV-B irradiation experiment in mouse skin, the sebaceous gland pairs was strongly implicated as a site for the generation of reactive oxygen species (ROS). In a histological evaluation of the skin stained with CH3MDFDA (ROS index) and YO-Pro-1 (apoptosis index), the fluorescence intensity of a sebaceous gland significantly increased with UV-B irradiation. With the application of fullerene to UV-irradiated mouse skin, no toxicity was recognized in comparison with the control, and erythema, the ROS index, and the apoptosis index decrease with the application of fullerene. Ascorbyl radical (AA{radical dot}) increased with the application of ascorbate (AA) to UV-B-irradiated mouse skin, and AA{radical dot} decreased with the application of fullerene. The co-application of AA and fullerene, which suppressed AA{radical dot} in vitro, significantly suppressed erythema, and also suppressed both the ROS index and apoptosis index in mouse skin after UV-B irradiation. In both mouse skin at 48 h after UV-B irradiation and in an attempt to reproduce this phenomenon artificially in vitro, a similar high AA{radical dot} peak (AA{radical dot}/H{radical dot} > 4) was observed in electron spin resonance (ESR) charts. The binding of fullerene with AA impairs the Fenton reaction between AA and Fe-protein based on the observation of ascorbate-specific UV absorption and a linear equation for the calibration curve. Therefore, fullerene may impair the intercalation of AA to a heme pocket by binding with AA. These results suggest that the co-application of AA and fullerene is effective against oxidative skin damage caused by UV-B irradiation, and the development of an AA{radical dot} inhibitor such as fullerene should be useful for reducing organ damage associated with Fe-protein oxidation.
AB - The role of fullerene as a pro-oxidant or anti-oxidant in Ultraviolet B ray (UV-B)-induced disorders in mouse skin was investigated. Fullerene gave no photo-toxic effect to UV-B-irradiated mouse skin. Since erythema was concentrated at the pore circumference in a UV-B irradiation experiment in mouse skin, the sebaceous gland pairs was strongly implicated as a site for the generation of reactive oxygen species (ROS). In a histological evaluation of the skin stained with CH3MDFDA (ROS index) and YO-Pro-1 (apoptosis index), the fluorescence intensity of a sebaceous gland significantly increased with UV-B irradiation. With the application of fullerene to UV-irradiated mouse skin, no toxicity was recognized in comparison with the control, and erythema, the ROS index, and the apoptosis index decrease with the application of fullerene. Ascorbyl radical (AA{radical dot}) increased with the application of ascorbate (AA) to UV-B-irradiated mouse skin, and AA{radical dot} decreased with the application of fullerene. The co-application of AA and fullerene, which suppressed AA{radical dot} in vitro, significantly suppressed erythema, and also suppressed both the ROS index and apoptosis index in mouse skin after UV-B irradiation. In both mouse skin at 48 h after UV-B irradiation and in an attempt to reproduce this phenomenon artificially in vitro, a similar high AA{radical dot} peak (AA{radical dot}/H{radical dot} > 4) was observed in electron spin resonance (ESR) charts. The binding of fullerene with AA impairs the Fenton reaction between AA and Fe-protein based on the observation of ascorbate-specific UV absorption and a linear equation for the calibration curve. Therefore, fullerene may impair the intercalation of AA to a heme pocket by binding with AA. These results suggest that the co-application of AA and fullerene is effective against oxidative skin damage caused by UV-B irradiation, and the development of an AA{radical dot} inhibitor such as fullerene should be useful for reducing organ damage associated with Fe-protein oxidation.
UR - http://www.scopus.com/inward/record.url?scp=71849094542&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=71849094542&partnerID=8YFLogxK
U2 - 10.1016/j.tox.2009.09.015
DO - 10.1016/j.tox.2009.09.015
M3 - Article
C2 - 19800932
AN - SCOPUS:71849094542
SN - 0300-483X
VL - 267
SP - 27
EP - 38
JO - Toxicology
JF - Toxicology
IS - 1-3
ER -