The co-application effects of fullerene and ascorbic acid on UV-B irradiated mouse skin

Shinobu Ito, Kazuyoshi Itoga, Masayuki Yamato, Hirohiko Akamatsu, Teruo Okano

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The role of fullerene as a pro-oxidant or anti-oxidant in Ultraviolet B ray (UV-B)-induced disorders in mouse skin was investigated. Fullerene gave no photo-toxic effect to UV-B-irradiated mouse skin. Since erythema was concentrated at the pore circumference in a UV-B irradiation experiment in mouse skin, the sebaceous gland pairs was strongly implicated as a site for the generation of reactive oxygen species (ROS). In a histological evaluation of the skin stained with CH3MDFDA (ROS index) and YO-Pro-1 (apoptosis index), the fluorescence intensity of a sebaceous gland significantly increased with UV-B irradiation. With the application of fullerene to UV-irradiated mouse skin, no toxicity was recognized in comparison with the control, and erythema, the ROS index, and the apoptosis index decrease with the application of fullerene. Ascorbyl radical (AA{radical dot}) increased with the application of ascorbate (AA) to UV-B-irradiated mouse skin, and AA{radical dot} decreased with the application of fullerene. The co-application of AA and fullerene, which suppressed AA{radical dot} in vitro, significantly suppressed erythema, and also suppressed both the ROS index and apoptosis index in mouse skin after UV-B irradiation. In both mouse skin at 48 h after UV-B irradiation and in an attempt to reproduce this phenomenon artificially in vitro, a similar high AA{radical dot} peak (AA{radical dot}/H{radical dot} > 4) was observed in electron spin resonance (ESR) charts. The binding of fullerene with AA impairs the Fenton reaction between AA and Fe-protein based on the observation of ascorbate-specific UV absorption and a linear equation for the calibration curve. Therefore, fullerene may impair the intercalation of AA to a heme pocket by binding with AA. These results suggest that the co-application of AA and fullerene is effective against oxidative skin damage caused by UV-B irradiation, and the development of an AA{radical dot} inhibitor such as fullerene should be useful for reducing organ damage associated with Fe-protein oxidation.

Original languageEnglish
Pages (from-to)27-38
Number of pages12
JournalToxicology
Volume267
Issue number1-3
DOIs
Publication statusPublished - 12-01-2010

All Science Journal Classification (ASJC) codes

  • Toxicology

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