The disposition and renal handling of enprofylline in endotoxemic rats by bacterial lipopolysaccharide (LPS)

M. Nadai, T. Hasegawa, K. Kato, L. Wang, Toshitaka Nabeshima, N. Kato

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Abstract

The effects of lipopolysaccharide (LPS), isolated from Klebsiella pneumoniae (O3:K1 - ), on the pharmacokinetic behavior and renal handling of enprofylline, which is mainly excreted into the urine by an active tubular secretion mechanism, were investigated in rats. LPS (50 and 250 μg/kg) was infused for 20 to 30 min 2 hr before an intravenous administration of enprofylline (2.5 mg/kg). LPS induced a decrease in the systemic clearance and an increase in the volume of distribution at the steady state of enprofylline without any histological changes in the kidneys. No changes in the protein-binding parameters of enprofylline were observed between the control and LPS-pretreated groups, although LPS slightly decreased the albumin concentration in plasma. LPS caused decreases in the apparent maximum capacity of transport (V(max)) from 71.24 to 15.02 μg/min, in the Michaelis- Menten constant (K(M)) from 3.04 to 1.42 μg/ml, and in the glomerular filtration rate as estimated for inulin clearance from 3.10 to 1.87 ml/min. These results indicate that LPS decreases both the affinity and capacity of the tubular transport system, and in turn decreases the tubular secretory intrinsic clearance of enprofylline as shown by V(max)/K(M). The mechanism for inducing changes in the pharmacokinetic behavior and renal handling of enprofylline by LPS may be related to the effects of LPS on tubular secretion of enprofylline and its distribution in the organs and peripheral tissues.

Original languageEnglish
Pages (from-to)611-616
Number of pages6
JournalDrug Metabolism and Disposition
Volume21
Issue number4
Publication statusPublished - 01-01-1993
Externally publishedYes

Fingerprint

Lipopolysaccharides
Kidney
Pharmacokinetics
enprofylline
Inulin
Klebsiella pneumoniae
Glomerular Filtration Rate
Protein Binding
Intravenous Administration
Albumins
Urine

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

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title = "The disposition and renal handling of enprofylline in endotoxemic rats by bacterial lipopolysaccharide (LPS)",
abstract = "The effects of lipopolysaccharide (LPS), isolated from Klebsiella pneumoniae (O3:K1 - ), on the pharmacokinetic behavior and renal handling of enprofylline, which is mainly excreted into the urine by an active tubular secretion mechanism, were investigated in rats. LPS (50 and 250 μg/kg) was infused for 20 to 30 min 2 hr before an intravenous administration of enprofylline (2.5 mg/kg). LPS induced a decrease in the systemic clearance and an increase in the volume of distribution at the steady state of enprofylline without any histological changes in the kidneys. No changes in the protein-binding parameters of enprofylline were observed between the control and LPS-pretreated groups, although LPS slightly decreased the albumin concentration in plasma. LPS caused decreases in the apparent maximum capacity of transport (V(max)) from 71.24 to 15.02 μg/min, in the Michaelis- Menten constant (K(M)) from 3.04 to 1.42 μg/ml, and in the glomerular filtration rate as estimated for inulin clearance from 3.10 to 1.87 ml/min. These results indicate that LPS decreases both the affinity and capacity of the tubular transport system, and in turn decreases the tubular secretory intrinsic clearance of enprofylline as shown by V(max)/K(M). The mechanism for inducing changes in the pharmacokinetic behavior and renal handling of enprofylline by LPS may be related to the effects of LPS on tubular secretion of enprofylline and its distribution in the organs and peripheral tissues.",
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The disposition and renal handling of enprofylline in endotoxemic rats by bacterial lipopolysaccharide (LPS). / Nadai, M.; Hasegawa, T.; Kato, K.; Wang, L.; Nabeshima, Toshitaka; Kato, N.

In: Drug Metabolism and Disposition, Vol. 21, No. 4, 01.01.1993, p. 611-616.

Research output: Contribution to journalArticle

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T1 - The disposition and renal handling of enprofylline in endotoxemic rats by bacterial lipopolysaccharide (LPS)

AU - Nadai, M.

AU - Hasegawa, T.

AU - Kato, K.

AU - Wang, L.

AU - Nabeshima, Toshitaka

AU - Kato, N.

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