TY - JOUR
T1 - The Distribution of Phosphatidylcholine Species in Superficial-Type Pharyngeal Carcinoma
AU - Ishikawa, Seiji
AU - Tateya, Ichiro
AU - Hayasaka, Takahiro
AU - Shinriki, Satoru
AU - Masaki, Noritaka
AU - Hirano, Shigeru
AU - Kitamura, Morimasa
AU - Muto, Manabu
AU - Morita, Shuko
AU - Setou, Mitsutoshi
AU - Ito, Juichi
N1 - Publisher Copyright:
© 2017 Seiji Ishikawa et al.
PY - 2017
Y1 - 2017
N2 - Objectives. Superficial-type pharyngeal squamous cell carcinoma (STPSCC) is defined as carcinoma in situ or microinvasive squamous cell carcinoma without invasion to the muscular layer. An exploration of the biological characteristics of STPSCC could uncover the invasion mechanism of this carcinoma. Phosphatidylcholine (PC) in combination with fatty acids is considered to play an important role in cell motility. Imaging mass spectrometry (IMS) is especially suitable for phospholipid analysis because this technique can distinguish even fatty acid compositions. Study Design. IMS analysis of frozen human specimens. Methods. IMS analysis was conducted to elucidate the distribution of PC species in STPSCC tissues. STPSCC tissue sections from five patients were analyzed, and we identified the signals that showed significant increases in the subepithelial invasive region relative to the superficial region. Results. Three kinds of PC species containing arachidonic acid, that is, PC (16:0/20:4), PC (18:1/20:4), and PC (18:0/20:4), were increased in the subepithelial invasive region. Conclusion. These results may be associated with the invasion mechanism of hypopharyngeal carcinoma.
AB - Objectives. Superficial-type pharyngeal squamous cell carcinoma (STPSCC) is defined as carcinoma in situ or microinvasive squamous cell carcinoma without invasion to the muscular layer. An exploration of the biological characteristics of STPSCC could uncover the invasion mechanism of this carcinoma. Phosphatidylcholine (PC) in combination with fatty acids is considered to play an important role in cell motility. Imaging mass spectrometry (IMS) is especially suitable for phospholipid analysis because this technique can distinguish even fatty acid compositions. Study Design. IMS analysis of frozen human specimens. Methods. IMS analysis was conducted to elucidate the distribution of PC species in STPSCC tissues. STPSCC tissue sections from five patients were analyzed, and we identified the signals that showed significant increases in the subepithelial invasive region relative to the superficial region. Results. Three kinds of PC species containing arachidonic acid, that is, PC (16:0/20:4), PC (18:1/20:4), and PC (18:0/20:4), were increased in the subepithelial invasive region. Conclusion. These results may be associated with the invasion mechanism of hypopharyngeal carcinoma.
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U2 - 10.1155/2017/5387913
DO - 10.1155/2017/5387913
M3 - Article
C2 - 28373982
AN - SCOPUS:85015959264
VL - 2017
JO - BioMed Research International
JF - BioMed Research International
SN - 2314-6133
M1 - 5387913
ER -