The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion

Noritoshi Kato, Yukio Yuzawa, Tomoki Kosugi, Akinori Hobo, Waichi Sato, Yuko Miwa, Kazuma Sakamoto, Seiichi Matsuo, Kenji Kadomatsu

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

E-selectin and its ligands are essential for extravasation of leukocytes in inflammation. Here, we report that basigin (Bsg)/CD147 is a ligand for E-selectin that promotes renal inflammation in ischemia/ reperfusion. Compared with wild-type mice, Bsg-deficient (Bsg-/-) mice demonstrated striking suppression of neutrophil infiltration in the kidney after renal ischemia/reperfusion. Although E-selectin expression increased similarly between the two genotypes, Bsg-/- mice exhibited less renal damage, suggesting that Bsg on neutrophils contribute to renal injury in this model. Neutrophils expressed Bsg with N-linked polylactosamine chains and Bsg -/- neutrophils showed reduced binding to E-selectin. Bsg isolated from HL-60 cells bound to E-selectin, and tunicamycin treatment to abolish N-linked glycans from Bsg abrogated this binding. Furthermore, Bsg-/- neutrophils exhibited reduced E-selectin-dependent adherence to human umbilical vein endothelial cells in vitro. Injection of labeled neutrophils into mice showed that Bsg-/- neutrophils were less readily recruited to the kidney after renal ischemia/ reperfusion than Bsg+/+ neutrophils, regardless of the recipient's genotype. Taken together, these results indicate that Bsg is a physiologic ligand for E-selectin that plays a critical role in the renal damage induced by ischemia/reperfusion.

Original languageEnglish
Pages (from-to)1565-1576
Number of pages12
JournalJournal of the American Society of Nephrology
Volume20
Issue number7
DOIs
Publication statusPublished - 01-07-2009

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CD147 Antigens
E-Selectin
Neutrophil Infiltration
Reperfusion
Ischemia
Ligands
Kidney
Neutrophils
Genotype
Inflammation
Tunicamycin
HL-60 Cells
Human Umbilical Vein Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Kato, Noritoshi ; Yuzawa, Yukio ; Kosugi, Tomoki ; Hobo, Akinori ; Sato, Waichi ; Miwa, Yuko ; Sakamoto, Kazuma ; Matsuo, Seiichi ; Kadomatsu, Kenji. / The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. In: Journal of the American Society of Nephrology. 2009 ; Vol. 20, No. 7. pp. 1565-1576.
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The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. / Kato, Noritoshi; Yuzawa, Yukio; Kosugi, Tomoki; Hobo, Akinori; Sato, Waichi; Miwa, Yuko; Sakamoto, Kazuma; Matsuo, Seiichi; Kadomatsu, Kenji.

In: Journal of the American Society of Nephrology, Vol. 20, No. 7, 01.07.2009, p. 1565-1576.

Research output: Contribution to journalArticle

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AU - Kato, Noritoshi

AU - Yuzawa, Yukio

AU - Kosugi, Tomoki

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AU - Sato, Waichi

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