TY - JOUR
T1 - The earliest stages of B cell development require a chemokine stromal cell-derived factor/pre-B cell growth-stimulating factor
AU - Egawa, Takeshi
AU - Kawabata, Kenji
AU - Kawamoto, Hiroshi
AU - Amada, Kei
AU - Okamoto, Rika
AU - Fujii, Nobutaka
AU - Kishimoto, Tadamitsu
AU - Katsura, Yoshimoto
AU - Nagasawa, Takashi
N1 - Funding Information:
We thank S.-I. Nishikawa for anti-IL-7Rα antibody, H. Kiyono for IL-7 −/− mice, and T. Usami, M. Komaki, and E. Miyade for technical assistance. This study was supported by a Research for the Future Program grant of The Japanese Society for the Promotion of Science and by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2001
Y1 - 2001
N2 - Environmental factors essential for the first stages of B lymphopoiesis remain elusive. Here, we report that immediately after commitment to B lineage, precursors become dependent on a chemokine SDF-1 and its receptor CXCR4 using mutant and radiation chimeric mice. In bone marrow, generation of the earliest identifiable B cell precursor populations requires CXCR4. In fetal liver, we identified Lin-CD19-c-kit+IL-7Rα+AA4. 1+, the earliest unipotent B cell precursor population, and found that its development was severely affected in SDF-1-/- embryos but not in IL-7-/- embryos. Lin- T cell progenitors appeared normal in SDF-1-/- embryos. Moreover, SDF-1 exhibited specific biologic activities on the earliest B cell precursors. SDF-1 provides the first example of a cytokine responsible for the earliest B lineage stages.
AB - Environmental factors essential for the first stages of B lymphopoiesis remain elusive. Here, we report that immediately after commitment to B lineage, precursors become dependent on a chemokine SDF-1 and its receptor CXCR4 using mutant and radiation chimeric mice. In bone marrow, generation of the earliest identifiable B cell precursor populations requires CXCR4. In fetal liver, we identified Lin-CD19-c-kit+IL-7Rα+AA4. 1+, the earliest unipotent B cell precursor population, and found that its development was severely affected in SDF-1-/- embryos but not in IL-7-/- embryos. Lin- T cell progenitors appeared normal in SDF-1-/- embryos. Moreover, SDF-1 exhibited specific biologic activities on the earliest B cell precursors. SDF-1 provides the first example of a cytokine responsible for the earliest B lineage stages.
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U2 - 10.1016/S1074-7613(01)00185-6
DO - 10.1016/S1074-7613(01)00185-6
M3 - Article
C2 - 11520466
AN - SCOPUS:0034877143
SN - 1074-7613
VL - 15
SP - 323
EP - 334
JO - Immunity
JF - Immunity
IS - 2
ER -
