The enhancing action of D-galactosamine on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells

Akiko Morikawa; Naoki Koide; Tsuyoshi Sugiyama; Mya Mya Mu; Ferdaus Hassan; Shamima Islam; Hiroyasu Ito; Isamu Mori; Tomoaki Yoshida; Takashi Yokochi

doi:10.1016/j.femsim.2004.03.008

The enhancing action of D-galactosamine on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells

Akiko Morikawa, Naoki Koide, Tsuyoshi Sugiyama, Mya Mya Mu, Ferdaus Hassan, Shamima Islam, Hiroyasu Ito, Isamu Mori, Tomoaki Yoshida, Takashi Yokochi

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The effect of D-galactosamine (D-GalN) on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells was examined. D-GalN augmented the production of NO, but not tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 cells. Pretreatment of D-GalN augmented the NO production whereas its post-treatment did not. D-GalN augmented the NO production in RAW 264.7 cells stimulated with either TNF-α and interferon-γ. The augmentation of LPS-induced NO production by D-GalN was due to enhanced expressions of an inducible type of NO synthase mRNA and proteins. Intracellular reactive oxygen species (ROS) were exclusively generated in RAW 264.7 cells stimulated with D-GalN and LPS. Scavenging of intracellular ROS abrogated the augmentation of NO production. It was therefore suggested that D-GalN might augment LPS-induced NO production through the generation of intracellular ROS.

Original language	English
Pages (from-to)	211-218
Number of pages	8
Journal	FEMS Immunology and Medical Microbiology
Volume	41
Issue number	3
DOIs	https://doi.org/10.1016/j.femsim.2004.03.008
Publication status	Published - 01-07-2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Microbiology
Immunology
Microbiology (medical)
Infectious Diseases

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10.1016/j.femsim.2004.03.008

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title = "The enhancing action of D-galactosamine on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells",

abstract = "The effect of D-galactosamine (D-GalN) on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells was examined. D-GalN augmented the production of NO, but not tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 cells. Pretreatment of D-GalN augmented the NO production whereas its post-treatment did not. D-GalN augmented the NO production in RAW 264.7 cells stimulated with either TNF-α and interferon-γ. The augmentation of LPS-induced NO production by D-GalN was due to enhanced expressions of an inducible type of NO synthase mRNA and proteins. Intracellular reactive oxygen species (ROS) were exclusively generated in RAW 264.7 cells stimulated with D-GalN and LPS. Scavenging of intracellular ROS abrogated the augmentation of NO production. It was therefore suggested that D-GalN might augment LPS-induced NO production through the generation of intracellular ROS.",

author = "Akiko Morikawa and Naoki Koide and Tsuyoshi Sugiyama and Mu, {Mya Mya} and Ferdaus Hassan and Shamima Islam and Hiroyasu Ito and Isamu Mori and Tomoaki Yoshida and Takashi Yokochi",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research (Kakenhi: 14570247) from the Ministry of Education, Science, Sports and Culture of Japan. We are grateful to K. Takahashi for the excellent technical assistance.",

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doi = "10.1016/j.femsim.2004.03.008",

language = "English",

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T1 - The enhancing action of D-galactosamine on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells

AU - Morikawa, Akiko

AU - Koide, Naoki

AU - Sugiyama, Tsuyoshi

AU - Mu, Mya Mya

AU - Hassan, Ferdaus

AU - Islam, Shamima

AU - Ito, Hiroyasu

AU - Mori, Isamu

AU - Yoshida, Tomoaki

AU - Yokochi, Takashi

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research (Kakenhi: 14570247) from the Ministry of Education, Science, Sports and Culture of Japan. We are grateful to K. Takahashi for the excellent technical assistance.

PY - 2004/7/1

Y1 - 2004/7/1

N2 - The effect of D-galactosamine (D-GalN) on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells was examined. D-GalN augmented the production of NO, but not tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 cells. Pretreatment of D-GalN augmented the NO production whereas its post-treatment did not. D-GalN augmented the NO production in RAW 264.7 cells stimulated with either TNF-α and interferon-γ. The augmentation of LPS-induced NO production by D-GalN was due to enhanced expressions of an inducible type of NO synthase mRNA and proteins. Intracellular reactive oxygen species (ROS) were exclusively generated in RAW 264.7 cells stimulated with D-GalN and LPS. Scavenging of intracellular ROS abrogated the augmentation of NO production. It was therefore suggested that D-GalN might augment LPS-induced NO production through the generation of intracellular ROS.

AB - The effect of D-galactosamine (D-GalN) on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells was examined. D-GalN augmented the production of NO, but not tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 cells. Pretreatment of D-GalN augmented the NO production whereas its post-treatment did not. D-GalN augmented the NO production in RAW 264.7 cells stimulated with either TNF-α and interferon-γ. The augmentation of LPS-induced NO production by D-GalN was due to enhanced expressions of an inducible type of NO synthase mRNA and proteins. Intracellular reactive oxygen species (ROS) were exclusively generated in RAW 264.7 cells stimulated with D-GalN and LPS. Scavenging of intracellular ROS abrogated the augmentation of NO production. It was therefore suggested that D-GalN might augment LPS-induced NO production through the generation of intracellular ROS.

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The enhancing action of D-galactosamine on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells

Abstract

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