The enhancing action of D-galactosamine on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells

Akiko Morikawa, Naoki Koide, Tsuyoshi Sugiyama, Mya Mya Mu, Ferdaus Hassan, Shamima Islam, Hiroyasu Ito, Isamu Mori, Tomoaki Yoshida, Takashi Yokochi

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The effect of D-galactosamine (D-GalN) on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells was examined. D-GalN augmented the production of NO, but not tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 cells. Pretreatment of D-GalN augmented the NO production whereas its post-treatment did not. D-GalN augmented the NO production in RAW 264.7 cells stimulated with either TNF-α and interferon-γ. The augmentation of LPS-induced NO production by D-GalN was due to enhanced expressions of an inducible type of NO synthase mRNA and proteins. Intracellular reactive oxygen species (ROS) were exclusively generated in RAW 264.7 cells stimulated with D-GalN and LPS. Scavenging of intracellular ROS abrogated the augmentation of NO production. It was therefore suggested that D-GalN might augment LPS-induced NO production through the generation of intracellular ROS.

Original languageEnglish
Pages (from-to)211-218
Number of pages8
JournalFEMS Immunology and Medical Microbiology
Volume41
Issue number3
DOIs
Publication statusPublished - 01-07-2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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