The enhancing action of D-galactosamine on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells

Akiko Morikawa; Naoki Koide; Tsuyoshi Sugiyama; Mya Mya Mu; Ferdaus Hassan; Shamima Islam; Hiroyasu Ito; Isamu Mori; Tomoaki Yoshida; Takashi Yokochi

doi:10.1016/j.femsim.2004.03.008

The enhancing action of D-galactosamine on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells

Akiko Morikawa
, Naoki Koide
, Tsuyoshi Sugiyama
, Mya Mya Mu
, Ferdaus Hassan
, Shamima Islam
, Hiroyasu Ito
, Isamu Mori
, Tomoaki Yoshida
, Takashi Yokochi

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The effect of D-galactosamine (D-GalN) on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells was examined. D-GalN augmented the production of NO, but not tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 cells. Pretreatment of D-GalN augmented the NO production whereas its post-treatment did not. D-GalN augmented the NO production in RAW 264.7 cells stimulated with either TNF-α and interferon-γ. The augmentation of LPS-induced NO production by D-GalN was due to enhanced expressions of an inducible type of NO synthase mRNA and proteins. Intracellular reactive oxygen species (ROS) were exclusively generated in RAW 264.7 cells stimulated with D-GalN and LPS. Scavenging of intracellular ROS abrogated the augmentation of NO production. It was therefore suggested that D-GalN might augment LPS-induced NO production through the generation of intracellular ROS.

Original language	English
Pages (from-to)	211-218
Number of pages	8
Journal	FEMS Immunology and Medical Microbiology
Volume	41
Issue number	3
DOIs	https://doi.org/10.1016/j.femsim.2004.03.008
Publication status	Published - 01-07-2004

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Microbiology
Immunology
Microbiology (medical)
Infectious Diseases

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10.1016/j.femsim.2004.03.008

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@article{d27325c6bc164e149b1a06b29c7fe1b0,

title = "The enhancing action of D-galactosamine on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells",

abstract = "The effect of D-galactosamine (D-GalN) on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells was examined. D-GalN augmented the production of NO, but not tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 cells. Pretreatment of D-GalN augmented the NO production whereas its post-treatment did not. D-GalN augmented the NO production in RAW 264.7 cells stimulated with either TNF-α and interferon-γ. The augmentation of LPS-induced NO production by D-GalN was due to enhanced expressions of an inducible type of NO synthase mRNA and proteins. Intracellular reactive oxygen species (ROS) were exclusively generated in RAW 264.7 cells stimulated with D-GalN and LPS. Scavenging of intracellular ROS abrogated the augmentation of NO production. It was therefore suggested that D-GalN might augment LPS-induced NO production through the generation of intracellular ROS.",

author = "Akiko Morikawa and Naoki Koide and Tsuyoshi Sugiyama and Mu, \{Mya Mya\} and Ferdaus Hassan and Shamima Islam and Hiroyasu Ito and Isamu Mori and Tomoaki Yoshida and Takashi Yokochi",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research (Kakenhi: 14570247) from the Ministry of Education, Science, Sports and Culture of Japan. We are grateful to K. Takahashi for the excellent technical assistance.",

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doi = "10.1016/j.femsim.2004.03.008",

language = "English",

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AU - Morikawa, Akiko

AU - Koide, Naoki

AU - Sugiyama, Tsuyoshi

AU - Mu, Mya Mya

AU - Hassan, Ferdaus

AU - Islam, Shamima

AU - Ito, Hiroyasu

AU - Mori, Isamu

AU - Yoshida, Tomoaki

AU - Yokochi, Takashi

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research (Kakenhi: 14570247) from the Ministry of Education, Science, Sports and Culture of Japan. We are grateful to K. Takahashi for the excellent technical assistance.

PY - 2004/7/1

Y1 - 2004/7/1

N2 - The effect of D-galactosamine (D-GalN) on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells was examined. D-GalN augmented the production of NO, but not tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 cells. Pretreatment of D-GalN augmented the NO production whereas its post-treatment did not. D-GalN augmented the NO production in RAW 264.7 cells stimulated with either TNF-α and interferon-γ. The augmentation of LPS-induced NO production by D-GalN was due to enhanced expressions of an inducible type of NO synthase mRNA and proteins. Intracellular reactive oxygen species (ROS) were exclusively generated in RAW 264.7 cells stimulated with D-GalN and LPS. Scavenging of intracellular ROS abrogated the augmentation of NO production. It was therefore suggested that D-GalN might augment LPS-induced NO production through the generation of intracellular ROS.

AB - The effect of D-galactosamine (D-GalN) on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells was examined. D-GalN augmented the production of NO, but not tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 cells. Pretreatment of D-GalN augmented the NO production whereas its post-treatment did not. D-GalN augmented the NO production in RAW 264.7 cells stimulated with either TNF-α and interferon-γ. The augmentation of LPS-induced NO production by D-GalN was due to enhanced expressions of an inducible type of NO synthase mRNA and proteins. Intracellular reactive oxygen species (ROS) were exclusively generated in RAW 264.7 cells stimulated with D-GalN and LPS. Scavenging of intracellular ROS abrogated the augmentation of NO production. It was therefore suggested that D-GalN might augment LPS-induced NO production through the generation of intracellular ROS.

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The enhancing action of D-galactosamine on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells

Abstract

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