The epidermal Integrin beta-1 and p75NTR positive cells proliferating and migrating during wound healing produce various growth factors, while the expression of p75NTR is decreased in patients with chronic skin ulcers

Yohei Iwata, Hirohiko Akamatsu, Seiji Hasegawa, Masayuki Takahashi, Akiko Yagami, Satoru Nakata, Kayoko Matsunaga

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Backgroud: More effective treatment strategies are needed for the chronic skin ulcers. Recently, it has been reported that clinical application of stem cells improve wound healing. Objective: We aimed to determine the dynamic time-course movement of epidermal stem cell markers especially p75 neurotrophin receptor (p75NTR) and Integrin beta-1 in wound healing process. Furthermore, we also investigated the presence of these markers in human. Methods: Epidermal Integrin beta-1 + and p75NTR + cells were counted in wound healing process in mice. Both cells were also counted in human skin specimen obtained from chronic skin ulcers and healthy controls. Growth factor gene expression levels by purified mouse epidermal p75NTR + cells were also analyzed using real-time RT-PCR. Results: Integrin beta-1 + and p75NTR + cells were proliferated from 3 days after wounding. Reepithelization was completed 7 days after wounding, and the numbers of cells were returned to the baseline levels by 14 days after wounding. Integrin beta-1 + cells were proliferated in the basal layer, and p75NTR + cells were proliferated in the upper layer of epidermis. In human skin, Integrin beta-1 + and p75NTR + cells were 81%±12% and 36%±15% of the basal cells, respectively. In patients with chronic skin ulcers, the percentage of Integrin beta-1 + cells in the epidermis was identical to healthy controls. Surprisingly, p75NTR + cells were significantly decreased in chronic skin ulcer patients (1.2%±2.6%; p<0.0005) compared to healthy controls. Purified mouse epidermal p75NTR + cells expressed higher transforming growth factor-beta2 and vascular endothelial growth factor-alpha transcripts and lower epidermal growth factor transcripts than p75NTR - cells. Conclusion: These results suggest that Integrin beta-1 + and p75NTR + cells play an important role in wound healing process, and that p75NTR may be a key molecule and a candidate for new therapeutic target besides preexisting molecules for chronic skin ulcer patients.

Original languageEnglish
Pages (from-to)122-129
Number of pages8
JournalJournal of Dermatological Science
Volume71
Issue number2
DOIs
Publication statusPublished - 01-08-2013

Fingerprint

Integrin beta Chains
Skin Ulcer
Nerve Growth Factor Receptor
Wound Healing
Intercellular Signaling Peptides and Proteins
Skin
Cells
Stem cells
Epidermis
Transforming Growth Factor beta2
Stem Cells
Molecules
Epidermal Growth Factor
Gene expression
Vascular Endothelial Growth Factor A

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

@article{f33f45222a0d47999824d2aa18e20d23,
title = "The epidermal Integrin beta-1 and p75NTR positive cells proliferating and migrating during wound healing produce various growth factors, while the expression of p75NTR is decreased in patients with chronic skin ulcers",
abstract = "Backgroud: More effective treatment strategies are needed for the chronic skin ulcers. Recently, it has been reported that clinical application of stem cells improve wound healing. Objective: We aimed to determine the dynamic time-course movement of epidermal stem cell markers especially p75 neurotrophin receptor (p75NTR) and Integrin beta-1 in wound healing process. Furthermore, we also investigated the presence of these markers in human. Methods: Epidermal Integrin beta-1 + and p75NTR + cells were counted in wound healing process in mice. Both cells were also counted in human skin specimen obtained from chronic skin ulcers and healthy controls. Growth factor gene expression levels by purified mouse epidermal p75NTR + cells were also analyzed using real-time RT-PCR. Results: Integrin beta-1 + and p75NTR + cells were proliferated from 3 days after wounding. Reepithelization was completed 7 days after wounding, and the numbers of cells were returned to the baseline levels by 14 days after wounding. Integrin beta-1 + cells were proliferated in the basal layer, and p75NTR + cells were proliferated in the upper layer of epidermis. In human skin, Integrin beta-1 + and p75NTR + cells were 81{\%}±12{\%} and 36{\%}±15{\%} of the basal cells, respectively. In patients with chronic skin ulcers, the percentage of Integrin beta-1 + cells in the epidermis was identical to healthy controls. Surprisingly, p75NTR + cells were significantly decreased in chronic skin ulcer patients (1.2{\%}±2.6{\%}; p<0.0005) compared to healthy controls. Purified mouse epidermal p75NTR + cells expressed higher transforming growth factor-beta2 and vascular endothelial growth factor-alpha transcripts and lower epidermal growth factor transcripts than p75NTR - cells. Conclusion: These results suggest that Integrin beta-1 + and p75NTR + cells play an important role in wound healing process, and that p75NTR may be a key molecule and a candidate for new therapeutic target besides preexisting molecules for chronic skin ulcer patients.",
author = "Yohei Iwata and Hirohiko Akamatsu and Seiji Hasegawa and Masayuki Takahashi and Akiko Yagami and Satoru Nakata and Kayoko Matsunaga",
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The epidermal Integrin beta-1 and p75NTR positive cells proliferating and migrating during wound healing produce various growth factors, while the expression of p75NTR is decreased in patients with chronic skin ulcers. / Iwata, Yohei; Akamatsu, Hirohiko; Hasegawa, Seiji; Takahashi, Masayuki; Yagami, Akiko; Nakata, Satoru; Matsunaga, Kayoko.

In: Journal of Dermatological Science, Vol. 71, No. 2, 01.08.2013, p. 122-129.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The epidermal Integrin beta-1 and p75NTR positive cells proliferating and migrating during wound healing produce various growth factors, while the expression of p75NTR is decreased in patients with chronic skin ulcers

AU - Iwata, Yohei

AU - Akamatsu, Hirohiko

AU - Hasegawa, Seiji

AU - Takahashi, Masayuki

AU - Yagami, Akiko

AU - Nakata, Satoru

AU - Matsunaga, Kayoko

PY - 2013/8/1

Y1 - 2013/8/1

N2 - Backgroud: More effective treatment strategies are needed for the chronic skin ulcers. Recently, it has been reported that clinical application of stem cells improve wound healing. Objective: We aimed to determine the dynamic time-course movement of epidermal stem cell markers especially p75 neurotrophin receptor (p75NTR) and Integrin beta-1 in wound healing process. Furthermore, we also investigated the presence of these markers in human. Methods: Epidermal Integrin beta-1 + and p75NTR + cells were counted in wound healing process in mice. Both cells were also counted in human skin specimen obtained from chronic skin ulcers and healthy controls. Growth factor gene expression levels by purified mouse epidermal p75NTR + cells were also analyzed using real-time RT-PCR. Results: Integrin beta-1 + and p75NTR + cells were proliferated from 3 days after wounding. Reepithelization was completed 7 days after wounding, and the numbers of cells were returned to the baseline levels by 14 days after wounding. Integrin beta-1 + cells were proliferated in the basal layer, and p75NTR + cells were proliferated in the upper layer of epidermis. In human skin, Integrin beta-1 + and p75NTR + cells were 81%±12% and 36%±15% of the basal cells, respectively. In patients with chronic skin ulcers, the percentage of Integrin beta-1 + cells in the epidermis was identical to healthy controls. Surprisingly, p75NTR + cells were significantly decreased in chronic skin ulcer patients (1.2%±2.6%; p<0.0005) compared to healthy controls. Purified mouse epidermal p75NTR + cells expressed higher transforming growth factor-beta2 and vascular endothelial growth factor-alpha transcripts and lower epidermal growth factor transcripts than p75NTR - cells. Conclusion: These results suggest that Integrin beta-1 + and p75NTR + cells play an important role in wound healing process, and that p75NTR may be a key molecule and a candidate for new therapeutic target besides preexisting molecules for chronic skin ulcer patients.

AB - Backgroud: More effective treatment strategies are needed for the chronic skin ulcers. Recently, it has been reported that clinical application of stem cells improve wound healing. Objective: We aimed to determine the dynamic time-course movement of epidermal stem cell markers especially p75 neurotrophin receptor (p75NTR) and Integrin beta-1 in wound healing process. Furthermore, we also investigated the presence of these markers in human. Methods: Epidermal Integrin beta-1 + and p75NTR + cells were counted in wound healing process in mice. Both cells were also counted in human skin specimen obtained from chronic skin ulcers and healthy controls. Growth factor gene expression levels by purified mouse epidermal p75NTR + cells were also analyzed using real-time RT-PCR. Results: Integrin beta-1 + and p75NTR + cells were proliferated from 3 days after wounding. Reepithelization was completed 7 days after wounding, and the numbers of cells were returned to the baseline levels by 14 days after wounding. Integrin beta-1 + cells were proliferated in the basal layer, and p75NTR + cells were proliferated in the upper layer of epidermis. In human skin, Integrin beta-1 + and p75NTR + cells were 81%±12% and 36%±15% of the basal cells, respectively. In patients with chronic skin ulcers, the percentage of Integrin beta-1 + cells in the epidermis was identical to healthy controls. Surprisingly, p75NTR + cells were significantly decreased in chronic skin ulcer patients (1.2%±2.6%; p<0.0005) compared to healthy controls. Purified mouse epidermal p75NTR + cells expressed higher transforming growth factor-beta2 and vascular endothelial growth factor-alpha transcripts and lower epidermal growth factor transcripts than p75NTR - cells. Conclusion: These results suggest that Integrin beta-1 + and p75NTR + cells play an important role in wound healing process, and that p75NTR may be a key molecule and a candidate for new therapeutic target besides preexisting molecules for chronic skin ulcer patients.

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