TY - JOUR
T1 - The Epstein-Barr virus BRRF2 gene product is involved in viral progeny production
AU - Watanabe, Takahiro
AU - Tsuruoka, Miyuki
AU - Narita, Yohei
AU - Katsuya, Ryotaro
AU - Goshima, Fumi
AU - Kimura, Hiroshi
AU - Murata, Takayuki
N1 - Funding Information:
We are grateful to Drs. T. Tsurumi, T. Kanda, S. Ohno, Y. Kawaguchi, W. Hammerschmidt, and H. J. Delecluse for scientific discussions and materials. This work was supported by Grants-in-aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (30470165 and 15K08494 to T.M. and 25293109 to H.K.) and the Ministry of Health, Labor and Welfare ( H26-Nanchi-013 to H.K.), and partly by the Uehara Memorial Research Fund, the Takeda Science Foundation, the Kanae Foundation for Promotion of Medical Science, the Kitamura Memorial Research Fund and the General Assembly of the Japanese Association of Medical Sciences (to T.M.).
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - The Epstein-Barr virus (EBV) predominantly establishes a latent infection in B lymphocytes, and occasionally switches from the latent state to the lytic cycle. In this report, we identified and examined the role of a lytic gene, BRRF2. We first prepared an antibody against BRRF2 and identified the gene product as a viral lytic protein expressed in B95-8 cells with late kinetics. Immunofluorescence revealed that BRRF2 localized in the cytoplasm of cells during the lytic phase. We also found that BRRF2 protein was phosphorylated in lytic cells, but the only viral protein kinase, BGLF4, was not involved in the phosphorylation. Knockout EBV and a repaired strain were then prepared, and we found that BRRF2 disruption did not affect viral gene expression and DNA replication, but decreased virus production. These results demonstrated that BRRF2 is involved in production of infectious progeny, although it is not essential for lytic replication.
AB - The Epstein-Barr virus (EBV) predominantly establishes a latent infection in B lymphocytes, and occasionally switches from the latent state to the lytic cycle. In this report, we identified and examined the role of a lytic gene, BRRF2. We first prepared an antibody against BRRF2 and identified the gene product as a viral lytic protein expressed in B95-8 cells with late kinetics. Immunofluorescence revealed that BRRF2 localized in the cytoplasm of cells during the lytic phase. We also found that BRRF2 protein was phosphorylated in lytic cells, but the only viral protein kinase, BGLF4, was not involved in the phosphorylation. Knockout EBV and a repaired strain were then prepared, and we found that BRRF2 disruption did not affect viral gene expression and DNA replication, but decreased virus production. These results demonstrated that BRRF2 is involved in production of infectious progeny, although it is not essential for lytic replication.
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U2 - 10.1016/j.virol.2015.05.010
DO - 10.1016/j.virol.2015.05.010
M3 - Article
C2 - 26057150
AN - SCOPUS:84930668171
VL - 484
SP - 33
EP - 40
JO - Virology
JF - Virology
SN - 0042-6822
ER -