Abstract
The Epstein-Barr virus (EBV) predominantly establishes a latent infection in B lymphocytes, and occasionally switches from the latent state to the lytic cycle. In this report, we identified and examined the role of a lytic gene, BRRF2. We first prepared an antibody against BRRF2 and identified the gene product as a viral lytic protein expressed in B95-8 cells with late kinetics. Immunofluorescence revealed that BRRF2 localized in the cytoplasm of cells during the lytic phase. We also found that BRRF2 protein was phosphorylated in lytic cells, but the only viral protein kinase, BGLF4, was not involved in the phosphorylation. Knockout EBV and a repaired strain were then prepared, and we found that BRRF2 disruption did not affect viral gene expression and DNA replication, but decreased virus production. These results demonstrated that BRRF2 is involved in production of infectious progeny, although it is not essential for lytic replication.
| Original language | English |
|---|---|
| Pages (from-to) | 33-40 |
| Number of pages | 8 |
| Journal | Virology |
| Volume | 484 |
| DOIs | |
| Publication status | Published - 01-10-2015 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Virology
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The Epstein-Barr virus BRRF2 gene product is involved in viral progeny production. / Watanabe, Takahiro; Tsuruoka, Miyuki; Narita, Yohei; Katsuya, Ryotaro; Goshima, Fumi; Kimura, Hiroshi; Murata, Takayuki.
In: Virology, Vol. 484, 01.10.2015, p. 33-40.Research output: Contribution to journal › Article
TY - JOUR
T1 - The Epstein-Barr virus BRRF2 gene product is involved in viral progeny production
AU - Watanabe, Takahiro
AU - Tsuruoka, Miyuki
AU - Narita, Yohei
AU - Katsuya, Ryotaro
AU - Goshima, Fumi
AU - Kimura, Hiroshi
AU - Murata, Takayuki
PY - 2015/10/1
Y1 - 2015/10/1
N2 - The Epstein-Barr virus (EBV) predominantly establishes a latent infection in B lymphocytes, and occasionally switches from the latent state to the lytic cycle. In this report, we identified and examined the role of a lytic gene, BRRF2. We first prepared an antibody against BRRF2 and identified the gene product as a viral lytic protein expressed in B95-8 cells with late kinetics. Immunofluorescence revealed that BRRF2 localized in the cytoplasm of cells during the lytic phase. We also found that BRRF2 protein was phosphorylated in lytic cells, but the only viral protein kinase, BGLF4, was not involved in the phosphorylation. Knockout EBV and a repaired strain were then prepared, and we found that BRRF2 disruption did not affect viral gene expression and DNA replication, but decreased virus production. These results demonstrated that BRRF2 is involved in production of infectious progeny, although it is not essential for lytic replication.
AB - The Epstein-Barr virus (EBV) predominantly establishes a latent infection in B lymphocytes, and occasionally switches from the latent state to the lytic cycle. In this report, we identified and examined the role of a lytic gene, BRRF2. We first prepared an antibody against BRRF2 and identified the gene product as a viral lytic protein expressed in B95-8 cells with late kinetics. Immunofluorescence revealed that BRRF2 localized in the cytoplasm of cells during the lytic phase. We also found that BRRF2 protein was phosphorylated in lytic cells, but the only viral protein kinase, BGLF4, was not involved in the phosphorylation. Knockout EBV and a repaired strain were then prepared, and we found that BRRF2 disruption did not affect viral gene expression and DNA replication, but decreased virus production. These results demonstrated that BRRF2 is involved in production of infectious progeny, although it is not essential for lytic replication.
UR - http://www.scopus.com/inward/record.url?scp=84930668171&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84930668171&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2015.05.010
DO - 10.1016/j.virol.2015.05.010
M3 - Article
C2 - 26057150
AN - SCOPUS:84930668171
VL - 484
SP - 33
EP - 40
JO - Virology
JF - Virology
SN - 0042-6822
ER -