The exocyst complex binds the small GTPase RalA to mediate filopodia formation

Kazuhiro Sugihara, Shiro Asano, Kenichi Tanaka, Akihiro Iwamatsu, Katsuya Okawa, Yasutaka Ohta

Research output: Contribution to journalArticle

196 Citations (Scopus)

Abstract

The Ras-related small GTPase RalA is involved in controlling actin cytoskeletal remodelling and vesicle transport in mammalian cells1, 2. We identified the mammalian homologue of Sec5, a subunit of the exocyst complex determining yeast cell polarity, as a specific binding partner for GTP-ligated RalA. Inhibition of RalA binding to Sec5 prevents filopod production by tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1) and by activated forms of RalA and Cdc42, signalling intermediates downstream of these inflammatory cytokines. We propose that the RalA-exocyst complex interaction integrates the secretory and cytoskeletal pathways.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalNature Cell Biology
Volume4
Issue number1
DOIs
Publication statusPublished - 01-01-2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology

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