The exon junction complex controls the efficient and faithful splicing of a subset of transcripts involved in mitotic cell-cycle progression

Kazuhiro Fukumura, Shunichi Wakabayashi, Naoyuki Kataoka, Hiroshi Sakamoto, Yutaka Suzuki, Kenta Nakai, Akira Maeda, Kunio Inoue

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon–exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However, a direct role for the human EJC in pre-mRNA splicing has not been fully understood. Using HeLa cells, we depleted one of the EJC core components, Y14, and the resulting transcriptome was analyzed by deep sequencing (RNA-Seq) and confirmed by RT–PCR. We found that Y14 is required for efficient and faithful splicing of a group of transcripts that is enriched in short intron-containing genes involved in mitotic cell-cycle progression. Tethering of EJC core components (Y14, eIF4AIII or MAGOH) to a model reporter pre-mRNA harboring a short intron showed that these core components are prerequisites for the splicing activation. Taken together, we conclude that the EJC core assembled on pre-mRNA is critical for efficient and faithful splicing of a specific subset of short introns in mitotic cell cycle-related genes.

Original languageEnglish
Article number1153
JournalInternational journal of molecular sciences
Volume17
Issue number8
DOIs
Publication statusPublished - 02-08-2016

Fingerprint

splicing
progressions
set theory
Exons
Cell Cycle
Cells
RNA Precursors
cycles
Introns
genes
Genes
High-Throughput Nucleotide Sequencing
cdc Genes
Messenger RNA
tethering
RNA
sequencing
HeLa Cells
Transcriptome
Gene expression

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Fukumura, Kazuhiro ; Wakabayashi, Shunichi ; Kataoka, Naoyuki ; Sakamoto, Hiroshi ; Suzuki, Yutaka ; Nakai, Kenta ; Maeda, Akira ; Inoue, Kunio. / The exon junction complex controls the efficient and faithful splicing of a subset of transcripts involved in mitotic cell-cycle progression. In: International journal of molecular sciences. 2016 ; Vol. 17, No. 8.
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The exon junction complex controls the efficient and faithful splicing of a subset of transcripts involved in mitotic cell-cycle progression. / Fukumura, Kazuhiro; Wakabayashi, Shunichi; Kataoka, Naoyuki; Sakamoto, Hiroshi; Suzuki, Yutaka; Nakai, Kenta; Maeda, Akira; Inoue, Kunio.

In: International journal of molecular sciences, Vol. 17, No. 8, 1153, 02.08.2016.

Research output: Contribution to journalArticle

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T1 - The exon junction complex controls the efficient and faithful splicing of a subset of transcripts involved in mitotic cell-cycle progression

AU - Fukumura, Kazuhiro

AU - Wakabayashi, Shunichi

AU - Kataoka, Naoyuki

AU - Sakamoto, Hiroshi

AU - Suzuki, Yutaka

AU - Nakai, Kenta

AU - Maeda, Akira

AU - Inoue, Kunio

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N2 - The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon–exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However, a direct role for the human EJC in pre-mRNA splicing has not been fully understood. Using HeLa cells, we depleted one of the EJC core components, Y14, and the resulting transcriptome was analyzed by deep sequencing (RNA-Seq) and confirmed by RT–PCR. We found that Y14 is required for efficient and faithful splicing of a group of transcripts that is enriched in short intron-containing genes involved in mitotic cell-cycle progression. Tethering of EJC core components (Y14, eIF4AIII or MAGOH) to a model reporter pre-mRNA harboring a short intron showed that these core components are prerequisites for the splicing activation. Taken together, we conclude that the EJC core assembled on pre-mRNA is critical for efficient and faithful splicing of a specific subset of short introns in mitotic cell cycle-related genes.

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