TY - JOUR
T1 - The exon junction complex controls the efficient and faithful splicing of a subset of transcripts involved in mitotic cell-cycle progression
AU - Fukumura, Kazuhiro
AU - Wakabayashi, Shunichi
AU - Kataoka, Naoyuki
AU - Sakamoto, Hiroshi
AU - Suzuki, Yutaka
AU - Nakai, Kenta
AU - Mayeda, Akila
AU - Inoue, Kunio
N1 - Publisher Copyright:
© 2016 by the authors; licensee MDPI, Basel, Switzerland.
PY - 2016/8/2
Y1 - 2016/8/2
N2 - The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon–exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However, a direct role for the human EJC in pre-mRNA splicing has not been fully understood. Using HeLa cells, we depleted one of the EJC core components, Y14, and the resulting transcriptome was analyzed by deep sequencing (RNA-Seq) and confirmed by RT–PCR. We found that Y14 is required for efficient and faithful splicing of a group of transcripts that is enriched in short intron-containing genes involved in mitotic cell-cycle progression. Tethering of EJC core components (Y14, eIF4AIII or MAGOH) to a model reporter pre-mRNA harboring a short intron showed that these core components are prerequisites for the splicing activation. Taken together, we conclude that the EJC core assembled on pre-mRNA is critical for efficient and faithful splicing of a specific subset of short introns in mitotic cell cycle-related genes.
AB - The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon–exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However, a direct role for the human EJC in pre-mRNA splicing has not been fully understood. Using HeLa cells, we depleted one of the EJC core components, Y14, and the resulting transcriptome was analyzed by deep sequencing (RNA-Seq) and confirmed by RT–PCR. We found that Y14 is required for efficient and faithful splicing of a group of transcripts that is enriched in short intron-containing genes involved in mitotic cell-cycle progression. Tethering of EJC core components (Y14, eIF4AIII or MAGOH) to a model reporter pre-mRNA harboring a short intron showed that these core components are prerequisites for the splicing activation. Taken together, we conclude that the EJC core assembled on pre-mRNA is critical for efficient and faithful splicing of a specific subset of short introns in mitotic cell cycle-related genes.
KW - Exon junction complex (EJC)
KW - Mitotic cell-cycle
KW - Pre-mRNA splicing
KW - Y14
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U2 - 10.3390/ijms17081153
DO - 10.3390/ijms17081153
M3 - Article
C2 - 27490541
AN - SCOPUS:84982696427
SN - 1661-6596
VL - 17
JO - International journal of molecular sciences
JF - International journal of molecular sciences
IS - 8
M1 - 1153
ER -