The expression of CD8α discriminates distinct T cell subsets in teleost fish

Fumio Takizawa, J・m Dijkstra, Paul Kotterba, Tomáš Korytář, Holger Kock, Bernd Köllner, Beltran Jaureguiberry, Teruyuki Nakanishi, Uwe Fischer

Research output: Contribution to journalArticle

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Abstract

CD8, belonging to the TCR complex, is the main marker molecule of CTLs. Although CD8 genes have been detected in many fish species, the analysis of teleost CD8+ cells has been limited because of the lack of antibodies. Using newly established mAbs against rainbow trout CD8α, we found high ratios of CD8α+ cells in trout thymus, gill and intestine, but relatively low abundance in pronephros, spleen and blood. Accordingly, tissue sections revealed many CD8α+ cells in thymus, numerous intra- and subepithelial CD8α+ cells in intestine and gill and few scattered CD8α+ cells in spleen and pronephros. In secondary lymphoid tissues, CD8α+ lymphocytes, which did not react with anti-thrombocyte or anti-IgM mAbs, expressed CD8α, CD8β and TCRα, while Ig and CD4 transcripts were found in CD8α- lymphocytes. In contrast, considerable CD4 expression in CD8α+ thymocytes suggests the presence of double-positive early T cells. Highly expressed TCRγ, LAG3 and CTLA4 in CD8α+ lymphocytes imply that they constitute a heterogeneous population different from found in non-mucosal tissues. PHA stimulation resulted in an up-regulation of CTL effector genes (perforin, granulysin and IFN-γ) in CD8α+ pronephrocytes, while both Th1 (IFN-γ) and Th2 (IL-4/13A) cytokines were up-regulated in CD8α- pronephrocytes. Although the basic characteristics of CD8α+ lymphocytes seem similar in teleost and mammals, features such as the low proportion of teleost CD8α+ lymphocytes in blood and their high abundance in respiratory tissue reveal a unique dynamics and distribution.

Original languageEnglish
Pages (from-to)752-763
Number of pages12
JournalDevelopmental and Comparative Immunology
Volume35
Issue number7
DOIs
Publication statusPublished - 01-07-2011

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T-Lymphocyte Subsets
Fishes
Lymphocytes
Pronephros
Thymus Gland
Intestines
Spleen
Perforin
Trout
Oncorhynchus mykiss
Lymphoid Tissue
Thymocytes
Interleukin-4
Genes
Mammals
Up-Regulation
Blood Platelets
Cytokines
T-Lymphocytes
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

Cite this

Takizawa, Fumio ; Dijkstra, J・m ; Kotterba, Paul ; Korytář, Tomáš ; Kock, Holger ; Köllner, Bernd ; Jaureguiberry, Beltran ; Nakanishi, Teruyuki ; Fischer, Uwe. / The expression of CD8α discriminates distinct T cell subsets in teleost fish. In: Developmental and Comparative Immunology. 2011 ; Vol. 35, No. 7. pp. 752-763.
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Takizawa, F, Dijkstra, J, Kotterba, P, Korytář, T, Kock, H, Köllner, B, Jaureguiberry, B, Nakanishi, T & Fischer, U 2011, 'The expression of CD8α discriminates distinct T cell subsets in teleost fish', Developmental and Comparative Immunology, vol. 35, no. 7, pp. 752-763. https://doi.org/10.1016/j.dci.2011.02.008

The expression of CD8α discriminates distinct T cell subsets in teleost fish. / Takizawa, Fumio; Dijkstra, J・m; Kotterba, Paul; Korytář, Tomáš; Kock, Holger; Köllner, Bernd; Jaureguiberry, Beltran; Nakanishi, Teruyuki; Fischer, Uwe.

In: Developmental and Comparative Immunology, Vol. 35, No. 7, 01.07.2011, p. 752-763.

Research output: Contribution to journalArticle

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T1 - The expression of CD8α discriminates distinct T cell subsets in teleost fish

AU - Takizawa, Fumio

AU - Dijkstra, J・m

AU - Kotterba, Paul

AU - Korytář, Tomáš

AU - Kock, Holger

AU - Köllner, Bernd

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AU - Nakanishi, Teruyuki

AU - Fischer, Uwe

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