Abstract
In the current study, we examined the cytoskeletal architecture of cod hepatic stellate cells. We found that the cod hepatic stellate cells contain abundant cytoplasmic filaments. Deep-etch electron microscopy showed that the major component of the cytoplasmic filaments was intermediate filaments, although microtubules and microfilaments were also found in the cytoplasmic filament bundles. Immunoelectron microscopy revealed the presence of βtubulin, α smooth muscle actin, smooth muscle type myosin, desmin and cytokeratin but not vimentin or glial fibrillar acidic protein. These results demonstrate that the cytoplasmic filaments of cod hepatic stellate cells are composed of desmin and cytokeratin intermediate filaments, acto-myosin complexes and microtubules, suggesting that the cod hepatic stellate cells have both contractile and structural functions. The expression of cytokeratin in cod hepatic stellate cells indicates that they serve for mechanical support in the extremely soft liver tissues of cods with their abundant lipids.
| Original language | English |
|---|---|
| Pages (from-to) | 437-444 |
| Number of pages | 8 |
| Journal | Archives of Histology and Cytology |
| Volume | 66 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 01-12-2003 |
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All Science Journal Classification (ASJC) codes
- Histology
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The Expression of cytokeratin in hepatic stellate cells of the cod. / Senda, Takao; Nomura, Ryuji.
In: Archives of Histology and Cytology, Vol. 66, No. 5, 01.12.2003, p. 437-444.Research output: Contribution to journal › Article
TY - JOUR
T1 - The Expression of cytokeratin in hepatic stellate cells of the cod
AU - Senda, Takao
AU - Nomura, Ryuji
PY - 2003/12/1
Y1 - 2003/12/1
N2 - In the current study, we examined the cytoskeletal architecture of cod hepatic stellate cells. We found that the cod hepatic stellate cells contain abundant cytoplasmic filaments. Deep-etch electron microscopy showed that the major component of the cytoplasmic filaments was intermediate filaments, although microtubules and microfilaments were also found in the cytoplasmic filament bundles. Immunoelectron microscopy revealed the presence of βtubulin, α smooth muscle actin, smooth muscle type myosin, desmin and cytokeratin but not vimentin or glial fibrillar acidic protein. These results demonstrate that the cytoplasmic filaments of cod hepatic stellate cells are composed of desmin and cytokeratin intermediate filaments, acto-myosin complexes and microtubules, suggesting that the cod hepatic stellate cells have both contractile and structural functions. The expression of cytokeratin in cod hepatic stellate cells indicates that they serve for mechanical support in the extremely soft liver tissues of cods with their abundant lipids.
AB - In the current study, we examined the cytoskeletal architecture of cod hepatic stellate cells. We found that the cod hepatic stellate cells contain abundant cytoplasmic filaments. Deep-etch electron microscopy showed that the major component of the cytoplasmic filaments was intermediate filaments, although microtubules and microfilaments were also found in the cytoplasmic filament bundles. Immunoelectron microscopy revealed the presence of βtubulin, α smooth muscle actin, smooth muscle type myosin, desmin and cytokeratin but not vimentin or glial fibrillar acidic protein. These results demonstrate that the cytoplasmic filaments of cod hepatic stellate cells are composed of desmin and cytokeratin intermediate filaments, acto-myosin complexes and microtubules, suggesting that the cod hepatic stellate cells have both contractile and structural functions. The expression of cytokeratin in cod hepatic stellate cells indicates that they serve for mechanical support in the extremely soft liver tissues of cods with their abundant lipids.
UR - http://www.scopus.com/inward/record.url?scp=1642504792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1642504792&partnerID=8YFLogxK
U2 - 10.1679/aohc.66.437
DO - 10.1679/aohc.66.437
M3 - Article
C2 - 15018146
AN - SCOPUS:1642504792
VL - 66
SP - 437
EP - 444
JO - Archives of Histology and Cytology
JF - Archives of Histology and Cytology
SN - 0914-9465
IS - 5
ER -