The Expression of cytokeratin in hepatic stellate cells of the cod

Takao Senda, Ryuji Nomura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In the current study, we examined the cytoskeletal architecture of cod hepatic stellate cells. We found that the cod hepatic stellate cells contain abundant cytoplasmic filaments. Deep-etch electron microscopy showed that the major component of the cytoplasmic filaments was intermediate filaments, although microtubules and microfilaments were also found in the cytoplasmic filament bundles. Immunoelectron microscopy revealed the presence of βtubulin, α smooth muscle actin, smooth muscle type myosin, desmin and cytokeratin but not vimentin or glial fibrillar acidic protein. These results demonstrate that the cytoplasmic filaments of cod hepatic stellate cells are composed of desmin and cytokeratin intermediate filaments, acto-myosin complexes and microtubules, suggesting that the cod hepatic stellate cells have both contractile and structural functions. The expression of cytokeratin in cod hepatic stellate cells indicates that they serve for mechanical support in the extremely soft liver tissues of cods with their abundant lipids.

Original languageEnglish
Pages (from-to)437-444
Number of pages8
JournalArchives of Histology and Cytology
Volume66
Issue number5
DOIs
Publication statusPublished - 01-12-2003

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Gadiformes
Hepatic Stellate Cells
Keratins
Cytoskeleton
Desmin
Intermediate Filaments
pioglitazone
Microtubules
Smooth Muscle Myosins
Immunoelectron Microscopy
Vimentin
Myosins
Tubulin
Actin Cytoskeleton
Neuroglia
Smooth Muscle
Actins
Electron Microscopy
Lipids
Liver

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

Cite this

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The Expression of cytokeratin in hepatic stellate cells of the cod. / Senda, Takao; Nomura, Ryuji.

In: Archives of Histology and Cytology, Vol. 66, No. 5, 01.12.2003, p. 437-444.

Research output: Contribution to journalArticle

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