Abstract
The surface expression of CD14 on mouse B-1 cells and its role on their response to lipopolysaccharide (LPS) were studied by using the murine TH2.52 B-1 cell line and peritoneal B-1 cells. TH2.52 cells with the B-1 phenotype were found to express membrane-bound CD14. Furthermore, CD14 was expressed on physiological peritoneal CD5+ B-1 cells. The stimulation of CD14-expressing TH2.52 cells with a low concentration of LPS resulted in the activation of nuclear factor (NF)-κB and a mitogen-activated protein kinase (MAPK). The LPS-induced NF-κB and MAPK activation was markedly inhibited by anti-CD14 antibody. These results suggest that B-1 cells may respond to LPS via membrane-bound CD14.
Original language | English |
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Pages (from-to) | 223-226 |
Number of pages | 4 |
Journal | Journal of Endotoxin Research |
Volume | 7 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2001 |
All Science Journal Classification (ASJC) codes
- Microbiology
- Immunology
- Molecular Biology
- Cell Biology
- Infectious Diseases