The expression of membrane-bound CD14 renders mouse B-1 cells susceptible to LPS

T. Sugiyama; N. Koide; D. Chakravortty; Y. Kato; M. M. Mu; T. Yoshida; T. Yokochi

doi:10.1179/096805101101532710

The expression of membrane-bound CD14 renders mouse B-1 cells susceptible to LPS

T. Sugiyama
, N. Koide
, D. Chakravortty
, Y. Kato
, M. M. Mu
, T. Yoshida
, T. Yokochi

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The surface expression of CD14 on mouse B-1 cells and its role on their response to lipopolysaccharide (LPS) were studied by using the murine TH2.52 B-1 cell line and peritoneal B-1 cells. TH2.52 cells with the B-1 phenotype were found to express membrane-bound CD14. Furthermore, CD14 was expressed on physiological peritoneal CD5⁺ B-1 cells. The stimulation of CD14-expressing TH2.52 cells with a low concentration of LPS resulted in the activation of nuclear factor (NF)-κB and a mitogen-activated protein kinase (MAPK). The LPS-induced NF-κB and MAPK activation was markedly inhibited by anti-CD14 antibody. These results suggest that B-1 cells may respond to LPS via membrane-bound CD14.

Original language	English
Pages (from-to)	223-226
Number of pages	4
Journal	Journal of Endotoxin Research
Volume	7
Issue number	3
DOIs	https://doi.org/10.1179/096805101101532710
Publication status	Published - 2001

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Molecular Biology
Cell Biology
Infectious Diseases

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10.1179/096805101101532710

Cite this

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title = "The expression of membrane-bound CD14 renders mouse B-1 cells susceptible to LPS",

abstract = "The surface expression of CD14 on mouse B-1 cells and its role on their response to lipopolysaccharide (LPS) were studied by using the murine TH2.52 B-1 cell line and peritoneal B-1 cells. TH2.52 cells with the B-1 phenotype were found to express membrane-bound CD14. Furthermore, CD14 was expressed on physiological peritoneal CD5+ B-1 cells. The stimulation of CD14-expressing TH2.52 cells with a low concentration of LPS resulted in the activation of nuclear factor (NF)-κB and a mitogen-activated protein kinase (MAPK). The LPS-induced NF-κB and MAPK activation was markedly inhibited by anti-CD14 antibody. These results suggest that B-1 cells may respond to LPS via membrane-bound CD14.",

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language = "English",

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T1 - The expression of membrane-bound CD14 renders mouse B-1 cells susceptible to LPS

AU - Sugiyama, T.

AU - Koide, N.

AU - Chakravortty, D.

AU - Kato, Y.

AU - Mu, M. M.

AU - Yoshida, T.

AU - Yokochi, T.

PY - 2001

Y1 - 2001

N2 - The surface expression of CD14 on mouse B-1 cells and its role on their response to lipopolysaccharide (LPS) were studied by using the murine TH2.52 B-1 cell line and peritoneal B-1 cells. TH2.52 cells with the B-1 phenotype were found to express membrane-bound CD14. Furthermore, CD14 was expressed on physiological peritoneal CD5+ B-1 cells. The stimulation of CD14-expressing TH2.52 cells with a low concentration of LPS resulted in the activation of nuclear factor (NF)-κB and a mitogen-activated protein kinase (MAPK). The LPS-induced NF-κB and MAPK activation was markedly inhibited by anti-CD14 antibody. These results suggest that B-1 cells may respond to LPS via membrane-bound CD14.

AB - The surface expression of CD14 on mouse B-1 cells and its role on their response to lipopolysaccharide (LPS) were studied by using the murine TH2.52 B-1 cell line and peritoneal B-1 cells. TH2.52 cells with the B-1 phenotype were found to express membrane-bound CD14. Furthermore, CD14 was expressed on physiological peritoneal CD5+ B-1 cells. The stimulation of CD14-expressing TH2.52 cells with a low concentration of LPS resulted in the activation of nuclear factor (NF)-κB and a mitogen-activated protein kinase (MAPK). The LPS-induced NF-κB and MAPK activation was markedly inhibited by anti-CD14 antibody. These results suggest that B-1 cells may respond to LPS via membrane-bound CD14.

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JO - Journal of Endotoxin Research

JF - Journal of Endotoxin Research

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The expression of membrane-bound CD14 renders mouse B-1 cells susceptible to LPS

Abstract

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