TY - JOUR
T1 - The human T-type amino acid transporter-1
T2 - Characterization, gene organization, and chromosomal location
AU - Kim, Do Kyung
AU - Kanai, Yoshikatsu
AU - Matsuo, Hirotaka
AU - Kim, Ju Young
AU - Chairoungdua, Arthit
AU - Kobayashi, Yukari
AU - Enomoto, Atsushi
AU - Cha, Seok Ho
AU - Goya, Tomoyuki
AU - Endou, Hitoshi
PY - 2002/1
Y1 - 2002/1
N2 - System T is a Na+-independent transport system that selectively transports aromatic amino acids. Here, we determined the structure of the human T-type amino-acid transporter-1 (TAT1) cDNA and gene (SLC16A1O). The human TAT1 cDNA encoded a 515-amino-acid protein with 12 putative membrane-spanning domains. Human SLC16A10 was localized on human chromosome 6, mapped to 6q21-q22. SLC16A10 contains six exons spanning 136 kb. In contrast to rat TAT1, which is mainly present in the intestine, human TAT1 was strongly expressed in human kidney as well as in human intestine. Expression of human TAT1 in Xenopus laevis oocytes demonstrated the Na+-independent transport of tryptophan, tyrosine, phenylalanine, and L-dopa, indicating that human TAT1 is a transporter subserving system T. Because human TAT1 is proposed to be crucial to the efficient absorption of aromatic amino acids from intestine and kidney, its defect could be involved in the disruption of aromatic amino-acid transport, such as in blue diaper syndrome.
AB - System T is a Na+-independent transport system that selectively transports aromatic amino acids. Here, we determined the structure of the human T-type amino-acid transporter-1 (TAT1) cDNA and gene (SLC16A1O). The human TAT1 cDNA encoded a 515-amino-acid protein with 12 putative membrane-spanning domains. Human SLC16A10 was localized on human chromosome 6, mapped to 6q21-q22. SLC16A10 contains six exons spanning 136 kb. In contrast to rat TAT1, which is mainly present in the intestine, human TAT1 was strongly expressed in human kidney as well as in human intestine. Expression of human TAT1 in Xenopus laevis oocytes demonstrated the Na+-independent transport of tryptophan, tyrosine, phenylalanine, and L-dopa, indicating that human TAT1 is a transporter subserving system T. Because human TAT1 is proposed to be crucial to the efficient absorption of aromatic amino acids from intestine and kidney, its defect could be involved in the disruption of aromatic amino-acid transport, such as in blue diaper syndrome.
UR - https://www.scopus.com/pages/publications/0035703933
UR - https://www.scopus.com/pages/publications/0035703933#tab=citedBy
U2 - 10.1006/geno.2001.6678
DO - 10.1006/geno.2001.6678
M3 - Article
C2 - 11827462
AN - SCOPUS:0035703933
SN - 0888-7543
VL - 79
SP - 95
EP - 103
JO - Genomics
JF - Genomics
IS - 1
ER -