The influence of beryllium on cell survival rate in the in-vitro culture system, on intracellular DNA synthesis and on SRBC-IgM antibody production responses

Tsutomu Yoshida, Syogo Shima, Hideki Kurita, Kaoru Nagaoka, Hiroshige Taniwaki, Yasuki Asada, Kai Ping Shai, Mitsumasa Koike, Kunihiko Morita

Research output: Contribution to journalArticlepeer-review

Abstract

Immunocytotoxicity of beryllium (Be) was evaluated by studying cell viability, intracellular DNA synthesis and SRBC-IgM response in an in-vitro culture system using non-sensitized spleen cells of a C57BL mouse. Be addition showed a suppressive effect on cell viability, an enhancing effect on DNA synthesis and on IgM antibody production. The suppressive effect on cell viability manifested itself markedly as the concentration of Be was increased or the culture time was prolonged. The DNA synthesis-enhancing effect was noted at a relatively low concentration of Be (not more than 10μM). The enhancing effect on the IgM response was related to Be concentration at not more than 20μM. The experimental results mentioned above speculate that the cytotoxicity of Be shows a conflicting pattern of enhancement or suppression according to the concentration used and that immunologically it has a modulating effect or an activating effect on the immunocompetent cells.

Original languageEnglish
Pages (from-to)117-121
Number of pages5
JournalEnvironmental Health and Preventive Medicine
Volume2
Issue number3
DOIs
Publication statusPublished - 10-1997

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health

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