TY - JOUR
T1 - The late effects of radiation on lifespan, lymphocyte proliferation and p53 haplodeficiency in mice
AU - Okazaki, Ryuji
AU - Mabuchi, Yo
AU - Yoshida, Yasuhiro
AU - Suzuki, Sadafumi
AU - Ding, Ning
AU - Matsuzaki, Yumi
AU - Ootsuyama, Akira
AU - Norimura, Toshiyuki
PY - 2010/11
Y1 - 2010/11
N2 - Purpose:We investigated the effect of irradiation on the lifespan of eight-week-old mice, the number of lymphocytes in bone marrow and the levels of p53 protein expression in the splenocytes. Methods and materials:Eight-week-old mice, wild-type p53 (p53+/) and heterozygous p53 (p53+/-), were irradiated with 3 Gy. The cell numbers and cell cycle phases of bone marrow cells were determined by flow cytometry. The splenocyte proliferation was evaluated by a fluorescent cell viability assay. The p53 expression was evaluated by Western blotting. Results:The lifespan of the irradiated mice was shorter than that of the non-irradiated mice. In irradiated 72-week-old p53 +/+ mice and 56-week-old p53+/- mice, the number of lymphocytes in bone marrow decreased as compared to that in the non-irradiated mice. In 56-week-old p53+/- mice, the S- and G2/M-phases of lymphocytes in the irradiated mice were increased compared to that in the non-irradiated mice. The splenocyte proliferation in p53+/+ mice decreased with age, and the proliferation in the irradiated mice was much lower than that in the non-irradiated mice. In 72-week-old p53+/+ mice after re-irradiation, the p53 protein expression in the splenocytes of the irradiated mice was delayed as compared to those from the non-irradiated mice. Conclusion:We suggest that the decrease in the number of lymphocytes in bone marrow and the delayed p53 expression in splenocytes from the irradiated mice are related to the shortened lifespan after irradiation at a young age.
AB - Purpose:We investigated the effect of irradiation on the lifespan of eight-week-old mice, the number of lymphocytes in bone marrow and the levels of p53 protein expression in the splenocytes. Methods and materials:Eight-week-old mice, wild-type p53 (p53+/) and heterozygous p53 (p53+/-), were irradiated with 3 Gy. The cell numbers and cell cycle phases of bone marrow cells were determined by flow cytometry. The splenocyte proliferation was evaluated by a fluorescent cell viability assay. The p53 expression was evaluated by Western blotting. Results:The lifespan of the irradiated mice was shorter than that of the non-irradiated mice. In irradiated 72-week-old p53 +/+ mice and 56-week-old p53+/- mice, the number of lymphocytes in bone marrow decreased as compared to that in the non-irradiated mice. In 56-week-old p53+/- mice, the S- and G2/M-phases of lymphocytes in the irradiated mice were increased compared to that in the non-irradiated mice. The splenocyte proliferation in p53+/+ mice decreased with age, and the proliferation in the irradiated mice was much lower than that in the non-irradiated mice. In 72-week-old p53+/+ mice after re-irradiation, the p53 protein expression in the splenocytes of the irradiated mice was delayed as compared to those from the non-irradiated mice. Conclusion:We suggest that the decrease in the number of lymphocytes in bone marrow and the delayed p53 expression in splenocytes from the irradiated mice are related to the shortened lifespan after irradiation at a young age.
UR - http://www.scopus.com/inward/record.url?scp=78049454142&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78049454142&partnerID=8YFLogxK
U2 - 10.3109/09553002.2010.496031
DO - 10.3109/09553002.2010.496031
M3 - Article
C2 - 20677904
AN - SCOPUS:78049454142
SN - 0955-3002
VL - 86
SP - 927
EP - 934
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 11
ER -