The loss of MCP-1 attenuates cutaneous ischemia-reperfusion injury in a mouse model of pressure ulcer

Yuki Saito, Minoru Hasegawa, Manabu Fujimoto, Takashi Matsushita, Mayuka Horikawa, Motoi Takenaka, Fumihide Ogawa, Junko Sugama, Douglas A. Steeber, Shinichi Sato, Kazuhiko Takehara

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

The formation of pressure ulcers is dependent on multiple factors including ischemia-reperfusion (IR). This study assessed the mechanism of a previously reported murine model of cutaneous IR injury. Three cycles of IR (days 1-3) by external application of two magnetic plates were performed to induce pressure ulcer formation. Increased infiltration of neutrophils and macrophages, and augmented expression of proinflammatory cytokines and inducible nitric oxide synthase (iNOS), were observed during IR cycles. In this model, monocyte chemoattractant protein-1 (MCP-1) was remarkably increased at day 1 in the skin followed by inflammatory cell infiltration. Therefore, IR cycles were performed in MCP-1-deficient (MCP-1-/-) mice to evaluate the role of this chemokine in pressure ulcer development. MCP-1-/- mice showed reduced macrophage infiltration and expression of tumor-necrosis factor-α (TNF)-α and iNOS during IR cycles leading to attenuated apoptosis and skin injury. Importantly, MCP-1 played a role in apoptosis and injury via inducing iNOS during the reperfusion rather than the ischemic period. These findings indicate that MCP-1 may be a critical factor for macrophage recruitment and subsequent skin inflammation and injury during IR cycles. We propose that this is a useful model for investigating the mechanism of pressure ulcer formation using various transgenic mice.

Original languageEnglish
Pages (from-to)1838-1851
Number of pages14
JournalJournal of Investigative Dermatology
Volume128
Issue number7
DOIs
Publication statusPublished - 07-2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Fingerprint

Dive into the research topics of 'The loss of MCP-1 attenuates cutaneous ischemia-reperfusion injury in a mouse model of pressure ulcer'. Together they form a unique fingerprint.

Cite this