The Major E6/E7 Transcript of HPV-16 in Exfoliated Cells from Cervical Neoplasia Patients

Takuma Fujii, Katsumi Tsukazaki, Kazushige Kiguchi, Kaneyuki Kubushiro, Masazumi Yajima, Shiro Nozawa

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

HPV-16 is implicated in the development of progressive cervical neoplasia, and E6 and E7 proteins of this virus play important roles in its oncogenic activity. Reverse transcriptase-nested-polymerase chain reaction (RT-nested PCR) method was applied to detect the major transcript (E6*I/E7) from E6/E7 ORFs of HPV-16 in the exfoliated cervical and vaginal cells from cervical neoplasia patients. The incidence of the E6*I/E7 transcript was proportional to the class of cytologic diagnosis. Further analysis revealed that the incidence of the E6*I/E7 transcript in Pap smears of class I or II was 5/28 (18%) patients who had previously been diagnosed as having cervical neoplasia but was 1/37 (3%) patients who had never been diagnosed (P < 0.05). These findings suggest that some patients followed up for cervical neoplasia were infected with transcriptionally active HPVs even when their Pap smears were negative. In addition, the E6*I/E7 transcript could not be detected after surgical treatment in any of the cases that were able to be followed up. The E6*I/E7 transcript in the exfoliated cells may prove valuable for epidemiological studies of the pathogenesis of HPV infection.

Original languageEnglish
Pages (from-to)210-215
Number of pages6
JournalGynecologic Oncology
Volume58
Issue number2
DOIs
Publication statusPublished - 01-01-1995
Externally publishedYes

Fingerprint

Human papillomavirus 16
Papanicolaou Test
Neoplasms
Incidence
Reverse Transcriptase Polymerase Chain Reaction
Open Reading Frames
Epidemiologic Studies
Viruses
Polymerase Chain Reaction
Infection
Proteins
Therapeutics

All Science Journal Classification (ASJC) codes

  • Oncology
  • Obstetrics and Gynaecology

Cite this

Fujii, Takuma ; Tsukazaki, Katsumi ; Kiguchi, Kazushige ; Kubushiro, Kaneyuki ; Yajima, Masazumi ; Nozawa, Shiro. / The Major E6/E7 Transcript of HPV-16 in Exfoliated Cells from Cervical Neoplasia Patients. In: Gynecologic Oncology. 1995 ; Vol. 58, No. 2. pp. 210-215.
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Fujii, T, Tsukazaki, K, Kiguchi, K, Kubushiro, K, Yajima, M & Nozawa, S 1995, 'The Major E6/E7 Transcript of HPV-16 in Exfoliated Cells from Cervical Neoplasia Patients', Gynecologic Oncology, vol. 58, no. 2, pp. 210-215. https://doi.org/10.1006/gyno.1995.1213

The Major E6/E7 Transcript of HPV-16 in Exfoliated Cells from Cervical Neoplasia Patients. / Fujii, Takuma; Tsukazaki, Katsumi; Kiguchi, Kazushige; Kubushiro, Kaneyuki; Yajima, Masazumi; Nozawa, Shiro.

In: Gynecologic Oncology, Vol. 58, No. 2, 01.01.1995, p. 210-215.

Research output: Contribution to journalArticle

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