The measurement of serum ceruloplasmin is useful for diagnostic differentiation of immune thrombocytopenic purpura

Mitsuhiro Chousa, Hiroyasu Ito, Kuniaki Saito, Kanako Takahashi, Masao Takemura, Takeshi Takahashi, Eiichi Tomita, Mitsuru Seishima

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: To expand the criteria of immune thrombocytopenic purpura (ITP), which is a diagnosis of exclusion, we analyzed proteins separated by 1-dimensional gel electrophoresis of the retained fraction in a Con A-Sepharose column from ITP patients' sera. Methods: Serum samples were from 19 adult patients with chronic ITP, 9 patients with thrombocytopenia of decreased production, and 20 healthy controls. Samples were applied to a Con A-Sepharose column, and the retained fraction was subjected to 10% SDS-PAGE. The % area of each densitometric protein peak was compared between the two groups and proteins in each band were identified using LC-MS/MS. Results: Eleven protein bands were distinctly separated by 1-dimensional electrophoresis. The percent area of bands #2 and #3 were significantly higher in ITP patients than in controls. The percent area of band #2 (p < 0.01) was significantly higher in ITP patients than in non-ITP patients. We identified α2-macroglobulin, ceruloplasmin (Cp), and C3 in band #2 and complement factor B in #3 band. Serum concentrations of α2-macroglobulin and Cp were significantly higher in ITP patients than in controls. Serum concentrations of Cp were significantly higher in ITP patients than in non-ITP patients (p = 0.0005). Serum complement factor B concentrations were significantly higher in ITP patients and non-ITP patients than in controls. ROC analysis showed that the total percent area of bands #2 and #3, and Cp had higher diagnosis availability for ITP patients when compared with controls and non-ITP patients, respectively. Conclusions: Measurement of Cp separated by the present method could be useful for the diagnosis of ITP in the presence of thrombocytopenia and a non- or low-inflammatory state.

Original languageEnglish
Pages (from-to)132-138
Number of pages7
JournalClinica Chimica Acta
Volume389
Issue number1-2
DOIs
Publication statusPublished - 01-03-2008
Externally publishedYes

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Ceruloplasmin
Idiopathic Thrombocytopenic Purpura
Macroglobulins
Serum
Complement Factor B
Thrombocytopenic Purpura
Electrophoresis
Proteins
Gels
Thrombocytopenia
Availability
ROC Curve
Polyacrylamide Gel Electrophoresis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Chousa, Mitsuhiro ; Ito, Hiroyasu ; Saito, Kuniaki ; Takahashi, Kanako ; Takemura, Masao ; Takahashi, Takeshi ; Tomita, Eiichi ; Seishima, Mitsuru. / The measurement of serum ceruloplasmin is useful for diagnostic differentiation of immune thrombocytopenic purpura. In: Clinica Chimica Acta. 2008 ; Vol. 389, No. 1-2. pp. 132-138.
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abstract = "Background: To expand the criteria of immune thrombocytopenic purpura (ITP), which is a diagnosis of exclusion, we analyzed proteins separated by 1-dimensional gel electrophoresis of the retained fraction in a Con A-Sepharose column from ITP patients' sera. Methods: Serum samples were from 19 adult patients with chronic ITP, 9 patients with thrombocytopenia of decreased production, and 20 healthy controls. Samples were applied to a Con A-Sepharose column, and the retained fraction was subjected to 10{\%} SDS-PAGE. The {\%} area of each densitometric protein peak was compared between the two groups and proteins in each band were identified using LC-MS/MS. Results: Eleven protein bands were distinctly separated by 1-dimensional electrophoresis. The percent area of bands #2 and #3 were significantly higher in ITP patients than in controls. The percent area of band #2 (p < 0.01) was significantly higher in ITP patients than in non-ITP patients. We identified α2-macroglobulin, ceruloplasmin (Cp), and C3 in band #2 and complement factor B in #3 band. Serum concentrations of α2-macroglobulin and Cp were significantly higher in ITP patients than in controls. Serum concentrations of Cp were significantly higher in ITP patients than in non-ITP patients (p = 0.0005). Serum complement factor B concentrations were significantly higher in ITP patients and non-ITP patients than in controls. ROC analysis showed that the total percent area of bands #2 and #3, and Cp had higher diagnosis availability for ITP patients when compared with controls and non-ITP patients, respectively. Conclusions: Measurement of Cp separated by the present method could be useful for the diagnosis of ITP in the presence of thrombocytopenia and a non- or low-inflammatory state.",
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Chousa, M, Ito, H, Saito, K, Takahashi, K, Takemura, M, Takahashi, T, Tomita, E & Seishima, M 2008, 'The measurement of serum ceruloplasmin is useful for diagnostic differentiation of immune thrombocytopenic purpura', Clinica Chimica Acta, vol. 389, no. 1-2, pp. 132-138. https://doi.org/10.1016/j.cca.2007.12.007

The measurement of serum ceruloplasmin is useful for diagnostic differentiation of immune thrombocytopenic purpura. / Chousa, Mitsuhiro; Ito, Hiroyasu; Saito, Kuniaki; Takahashi, Kanako; Takemura, Masao; Takahashi, Takeshi; Tomita, Eiichi; Seishima, Mitsuru.

In: Clinica Chimica Acta, Vol. 389, No. 1-2, 01.03.2008, p. 132-138.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The measurement of serum ceruloplasmin is useful for diagnostic differentiation of immune thrombocytopenic purpura

AU - Chousa, Mitsuhiro

AU - Ito, Hiroyasu

AU - Saito, Kuniaki

AU - Takahashi, Kanako

AU - Takemura, Masao

AU - Takahashi, Takeshi

AU - Tomita, Eiichi

AU - Seishima, Mitsuru

PY - 2008/3/1

Y1 - 2008/3/1

N2 - Background: To expand the criteria of immune thrombocytopenic purpura (ITP), which is a diagnosis of exclusion, we analyzed proteins separated by 1-dimensional gel electrophoresis of the retained fraction in a Con A-Sepharose column from ITP patients' sera. Methods: Serum samples were from 19 adult patients with chronic ITP, 9 patients with thrombocytopenia of decreased production, and 20 healthy controls. Samples were applied to a Con A-Sepharose column, and the retained fraction was subjected to 10% SDS-PAGE. The % area of each densitometric protein peak was compared between the two groups and proteins in each band were identified using LC-MS/MS. Results: Eleven protein bands were distinctly separated by 1-dimensional electrophoresis. The percent area of bands #2 and #3 were significantly higher in ITP patients than in controls. The percent area of band #2 (p < 0.01) was significantly higher in ITP patients than in non-ITP patients. We identified α2-macroglobulin, ceruloplasmin (Cp), and C3 in band #2 and complement factor B in #3 band. Serum concentrations of α2-macroglobulin and Cp were significantly higher in ITP patients than in controls. Serum concentrations of Cp were significantly higher in ITP patients than in non-ITP patients (p = 0.0005). Serum complement factor B concentrations were significantly higher in ITP patients and non-ITP patients than in controls. ROC analysis showed that the total percent area of bands #2 and #3, and Cp had higher diagnosis availability for ITP patients when compared with controls and non-ITP patients, respectively. Conclusions: Measurement of Cp separated by the present method could be useful for the diagnosis of ITP in the presence of thrombocytopenia and a non- or low-inflammatory state.

AB - Background: To expand the criteria of immune thrombocytopenic purpura (ITP), which is a diagnosis of exclusion, we analyzed proteins separated by 1-dimensional gel electrophoresis of the retained fraction in a Con A-Sepharose column from ITP patients' sera. Methods: Serum samples were from 19 adult patients with chronic ITP, 9 patients with thrombocytopenia of decreased production, and 20 healthy controls. Samples were applied to a Con A-Sepharose column, and the retained fraction was subjected to 10% SDS-PAGE. The % area of each densitometric protein peak was compared between the two groups and proteins in each band were identified using LC-MS/MS. Results: Eleven protein bands were distinctly separated by 1-dimensional electrophoresis. The percent area of bands #2 and #3 were significantly higher in ITP patients than in controls. The percent area of band #2 (p < 0.01) was significantly higher in ITP patients than in non-ITP patients. We identified α2-macroglobulin, ceruloplasmin (Cp), and C3 in band #2 and complement factor B in #3 band. Serum concentrations of α2-macroglobulin and Cp were significantly higher in ITP patients than in controls. Serum concentrations of Cp were significantly higher in ITP patients than in non-ITP patients (p = 0.0005). Serum complement factor B concentrations were significantly higher in ITP patients and non-ITP patients than in controls. ROC analysis showed that the total percent area of bands #2 and #3, and Cp had higher diagnosis availability for ITP patients when compared with controls and non-ITP patients, respectively. Conclusions: Measurement of Cp separated by the present method could be useful for the diagnosis of ITP in the presence of thrombocytopenia and a non- or low-inflammatory state.

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