TY - JOUR
T1 - The NLRP3 inflammasome – interleukin 1β axis in uveal melanoma
AU - Correa, Victor S.M.C.
AU - Efstathiou, Nikolaos E.
AU - Ntentakis, Dimitrios P.
AU - Yu, Zhen
AU - Narimatsu, Toshio
AU - Gragoudas, Evangelos
AU - Kim, Ivana K.
AU - Vavvas, Demetrios G.
N1 - Publisher Copyright:
© 2023 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
PY - 2023/3
Y1 - 2023/3
N2 - Uveal melanoma (UM) is the most common primary intraocular cancer in the adult population. Recent studies suggested that the NLRP3 inflammasome could be a therapeutic target for cutaneous melanoma (CM), but the role of NLRP3 in UM remains unknown. Here, we analyzed the NLRP3-IL-1β axis in 5 UM and 4 CM cell lines. Expression of NLRP3 mRNA in UM and CM was low, and expression in UM was lower than in CM (P < 0.001). NLRP3 protein levels were below detection limit for all cell lines. UM exhibited lower baseline IL-1β secretion than CM, especially when compared to the Hs294t cell line (P < 0.05). Bioinformatic analysis of human tumor samples showed that UM has significantly lower expression of NLRP3 and IL-1β compared with CM. In conclusion, our work shows evidence of extremely low NLRP3 expression and IL-1β secretion by melanoma cells and highlight differences between CM and UM.
AB - Uveal melanoma (UM) is the most common primary intraocular cancer in the adult population. Recent studies suggested that the NLRP3 inflammasome could be a therapeutic target for cutaneous melanoma (CM), but the role of NLRP3 in UM remains unknown. Here, we analyzed the NLRP3-IL-1β axis in 5 UM and 4 CM cell lines. Expression of NLRP3 mRNA in UM and CM was low, and expression in UM was lower than in CM (P < 0.001). NLRP3 protein levels were below detection limit for all cell lines. UM exhibited lower baseline IL-1β secretion than CM, especially when compared to the Hs294t cell line (P < 0.05). Bioinformatic analysis of human tumor samples showed that UM has significantly lower expression of NLRP3 and IL-1β compared with CM. In conclusion, our work shows evidence of extremely low NLRP3 expression and IL-1β secretion by melanoma cells and highlight differences between CM and UM.
KW - IL-1
KW - inflammasome
KW - melanoma
KW - NLRP3
KW - skin
KW - uveal
UR - https://www.scopus.com/pages/publications/85147916595
UR - https://www.scopus.com/pages/publications/85147916595#tab=citedBy
U2 - 10.1002/2211-5463.13566
DO - 10.1002/2211-5463.13566
M3 - Article
C2 - 36707938
AN - SCOPUS:85147916595
SN - 2211-5463
VL - 13
SP - 545
EP - 555
JO - FEBS Open Bio
JF - FEBS Open Bio
IS - 3
ER -
