The occurrence of mouse-type oligosaccharides in mouse-human chimeric immunoglobulin G

Taei Matsui, Jiharu Hamako, Koh zoh Kameyama, Yoshikazu Kurosawa, Koiti Titani, Tsuguo Mizuochi

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The asparagine-linked sugar chains of mouse-human chimeric IgG which is composed of the variable regions derived from mouse and the constant regions derived from human and produced in mouse cells were quantitatively released as tritium-labelled oligosaccharides by hydrazinolysis followed by N-acetylation and NaB3H4-reduction. Paper electrophoresis in combination with sialidase digestion indicated that more than 70% of the oligosaccharides were neutral and the rest was sialylated oligosaccharides. Their structures were determined by sequential exoglycosidase digestions. The mouse-human chimeric IgG was shown to have same sugar chains as those of mouse IgG.

Original languageEnglish
Pages (from-to)245-250
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume164
Issue number1
DOIs
Publication statusPublished - 16-10-1989

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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