The ontogeny of MHC class I expression in rainbow trout (Oncorhynchus mykiss)

Uwe Fischer, J・m Dijkstra, Bernd Köllner, Ikunari Kiryu, Erling Olav Koppang, Ivar Hordvik, Yoshihiro Sawamoto, Mitsuru Ototake

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In the present study, clonal rainbow trout (Oncorhynchus mykiss) embryos and larvae were assayed for the expression of key molecules involved in specific cell-mediated cytotoxicity using an anti-MHC class I monoclonal Ab and by RT-PCR using specific primers derived from classical MHC class I (class Ia), TCR and CD8. Whereas RT-PCR revealed that MHC class Ia and CD8 were expressed from at least 1 week after fertilisation (p.f.) on, TCR expression was detectable from 2 weeks p.f. Immunohistochemistry indicated an early and distinct expression of MHC class I protein in the thymus. Positive lymphoid, epithelial and endothelial cells were found in the pronephros, in the spleen and in the inner and outer epithelia at later stages. Whereas in older rainbow trout the intestine is counted among the organs of the highest class I expression, during ontogeny it was the last site (39 days after hatching) where such expression was detectable. Knowledge on the appearance of the assayed key molecules during fish development is relevant for the pathogenesis of infections as well as for early vaccine delivery. Besides such information regarding the development of the adaptive immune system, immunohistochemistry revealed that in early larvae MHC class I was expressed in neurons whereas in older rainbow trout this was not observed.

Original languageEnglish
Pages (from-to)49-60
Number of pages12
JournalFish and Shellfish Immunology
Volume18
Issue number1
DOIs
Publication statusPublished - 01-01-2005

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Oncorhynchus mykiss
ontogeny
rainbow
Thymus
Molecules
Immune system
Endothelial cells
Cytotoxicity
Fish
Neurons
larva
immunohistochemistry
Larva
Pronephros
Vaccines
Immunohistochemistry
reverse transcriptase polymerase chain reaction
vaccine
immune system
fish development

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

Fischer, U., Dijkstra, J., Köllner, B., Kiryu, I., Koppang, E. O., Hordvik, I., ... Ototake, M. (2005). The ontogeny of MHC class I expression in rainbow trout (Oncorhynchus mykiss). Fish and Shellfish Immunology, 18(1), 49-60. https://doi.org/10.1016/j.fsi.2004.05.006
Fischer, Uwe ; Dijkstra, J・m ; Köllner, Bernd ; Kiryu, Ikunari ; Koppang, Erling Olav ; Hordvik, Ivar ; Sawamoto, Yoshihiro ; Ototake, Mitsuru. / The ontogeny of MHC class I expression in rainbow trout (Oncorhynchus mykiss). In: Fish and Shellfish Immunology. 2005 ; Vol. 18, No. 1. pp. 49-60.
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Fischer, U, Dijkstra, J, Köllner, B, Kiryu, I, Koppang, EO, Hordvik, I, Sawamoto, Y & Ototake, M 2005, 'The ontogeny of MHC class I expression in rainbow trout (Oncorhynchus mykiss)', Fish and Shellfish Immunology, vol. 18, no. 1, pp. 49-60. https://doi.org/10.1016/j.fsi.2004.05.006

The ontogeny of MHC class I expression in rainbow trout (Oncorhynchus mykiss). / Fischer, Uwe; Dijkstra, J・m; Köllner, Bernd; Kiryu, Ikunari; Koppang, Erling Olav; Hordvik, Ivar; Sawamoto, Yoshihiro; Ototake, Mitsuru.

In: Fish and Shellfish Immunology, Vol. 18, No. 1, 01.01.2005, p. 49-60.

Research output: Contribution to journalArticle

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