The presence of catechol-o-methyltransferase activity in separately cultured cerebromicrovascular endothelial and smooth muscle cells

M. Spatz; N. Kaneda; C. Sumi; I. Nagatsu; C. R. Creveling; T. Nagatsu

doi:10.1016/0006-8993(86)90090-9

The presence of catechol-o-methyltransferase activity in separately cultured cerebromicrovascular endothelial and smooth muscle cells

M. Spatz, N. Kaneda, C. Sumi, I. Nagatsu, C. R. Creveling, T. Nagatsu

Department of Pharmacology

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Abstract

The activity of catechol-o-methyltransferase (COMT) was investigated in cultured and propagated cerebromicrovascular endothelial and smooth muscle cells using high performance liquid chromatography and immunocytochemistry. The existence of COMT was detected in both cell types. The demonstration of this enzyme activity in the cerebromicrovascular smooth muscle cells, in addition to the endothelium, indicates that the enzymatic barrier to catecholamine is not limited to capillaries, the main constituents of the blood-brain barrier.

Original language	English
Pages (from-to)	363-367
Number of pages	5
Journal	Brain Research
Volume	381
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(86)90090-9
Publication status	Published - 03-09-1986

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All Science Journal Classification (ASJC) codes

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

Cite this

Spatz, M., Kaneda, N., Sumi, C., Nagatsu, I., Creveling, C. R., & Nagatsu, T. (1986). The presence of catechol-o-methyltransferase activity in separately cultured cerebromicrovascular endothelial and smooth muscle cells. Brain Research, 381(2), 363-367. https://doi.org/10.1016/0006-8993(86)90090-9

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AU - Nagatsu, T.

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10.1016/0006-8993(86)90090-9