Abstract
The activity of catechol-o-methyltransferase (COMT) was investigated in cultured and propagated cerebromicrovascular endothelial and smooth muscle cells using high performance liquid chromatography and immunocytochemistry. The existence of COMT was detected in both cell types. The demonstration of this enzyme activity in the cerebromicrovascular smooth muscle cells, in addition to the endothelium, indicates that the enzymatic barrier to catecholamine is not limited to capillaries, the main constituents of the blood-brain barrier.
| Original language | English |
|---|---|
| Pages (from-to) | 363-367 |
| Number of pages | 5 |
| Journal | Brain Research |
| Volume | 381 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 03-09-1986 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology
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The presence of catechol-o-methyltransferase activity in separately cultured cerebromicrovascular endothelial and smooth muscle cells. / Spatz, M.; Kaneda, N.; Sumi, C.; Nagatsu, I.; Creveling, C. R.; Nagatsu, T.
In: Brain Research, Vol. 381, No. 2, 03.09.1986, p. 363-367.Research output: Contribution to journal › Article
TY - JOUR
T1 - The presence of catechol-o-methyltransferase activity in separately cultured cerebromicrovascular endothelial and smooth muscle cells
AU - Spatz, M.
AU - Kaneda, N.
AU - Sumi, C.
AU - Nagatsu, I.
AU - Creveling, C. R.
AU - Nagatsu, T.
PY - 1986/9/3
Y1 - 1986/9/3
N2 - The activity of catechol-o-methyltransferase (COMT) was investigated in cultured and propagated cerebromicrovascular endothelial and smooth muscle cells using high performance liquid chromatography and immunocytochemistry. The existence of COMT was detected in both cell types. The demonstration of this enzyme activity in the cerebromicrovascular smooth muscle cells, in addition to the endothelium, indicates that the enzymatic barrier to catecholamine is not limited to capillaries, the main constituents of the blood-brain barrier.
AB - The activity of catechol-o-methyltransferase (COMT) was investigated in cultured and propagated cerebromicrovascular endothelial and smooth muscle cells using high performance liquid chromatography and immunocytochemistry. The existence of COMT was detected in both cell types. The demonstration of this enzyme activity in the cerebromicrovascular smooth muscle cells, in addition to the endothelium, indicates that the enzymatic barrier to catecholamine is not limited to capillaries, the main constituents of the blood-brain barrier.
UR - http://www.scopus.com/inward/record.url?scp=0022492532&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022492532&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(86)90090-9
DO - 10.1016/0006-8993(86)90090-9
M3 - Article
C2 - 3530379
AN - SCOPUS:0022492532
VL - 381
SP - 363
EP - 367
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 2
ER -