TY - JOUR
T1 - The presence of catechol-o-methyltransferase activity in separately cultured cerebromicrovascular endothelial and smooth muscle cells
AU - Spatz, M.
AU - Kaneda, N.
AU - Sumi, C.
AU - Nagatsu, I.
AU - Creveling, C. R.
AU - Nagatsu, T.
PY - 1986/9/3
Y1 - 1986/9/3
N2 - The activity of catechol-o-methyltransferase (COMT) was investigated in cultured and propagated cerebromicrovascular endothelial and smooth muscle cells using high performance liquid chromatography and immunocytochemistry. The existence of COMT was detected in both cell types. The demonstration of this enzyme activity in the cerebromicrovascular smooth muscle cells, in addition to the endothelium, indicates that the enzymatic barrier to catecholamine is not limited to capillaries, the main constituents of the blood-brain barrier.
AB - The activity of catechol-o-methyltransferase (COMT) was investigated in cultured and propagated cerebromicrovascular endothelial and smooth muscle cells using high performance liquid chromatography and immunocytochemistry. The existence of COMT was detected in both cell types. The demonstration of this enzyme activity in the cerebromicrovascular smooth muscle cells, in addition to the endothelium, indicates that the enzymatic barrier to catecholamine is not limited to capillaries, the main constituents of the blood-brain barrier.
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U2 - 10.1016/0006-8993(86)90090-9
DO - 10.1016/0006-8993(86)90090-9
M3 - Article
C2 - 3530379
AN - SCOPUS:0022492532
VL - 381
SP - 363
EP - 367
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 2
ER -