The relationship between Helicobacter pylori infection and promoter polymorphism of the Nrf2 gene in chronic gastritis

Tomiyasu Arisawa, Tomomitsu Tahara, Tomoyuki Shibata, Mitsuo Nagasaka, Masakatsu Nakamura, Yoshio Kamiya, Hiroshi Fujita, Shin Hasegawa, Tamaki Takagi, Fang Yu Wang, Ichiro Hirata, Hiroshi Nakano

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Abstract

The transcription factor Nrf2 regulates the expression of detoxifying and antioxidant genes. Three polymorphisms of the Nrf2 gene have been reported. We attempted to clarify the relationship between Nrf2 gene polymorphism and chronic gastritis in a Japanese population. The study was performed in 159 patients with no evidence of gastric malignancy on upper gastrointestinal endoscopy (mean age, 62.03 years; male:female ratio, 102:57; peptic ulcer diseases in 69 patients, and Helicobacter pylori (H. pylori) positivity in 73.0%). We employed the PCR-SSCP method to detect gene polymorphisms using DNA extracted from peripheral blood cells or from antral biopsy specimens obtained by endoscopy. The severity of the histological chronic gastritis in antral biopsy specimens was classified according to the updated Sydney system. Although the frequencies of the SNP(-686) and SNP(-650) A alleles were decreased in subjects with peptic ulcers or severe mucosal atrophy, no significant differences were seen. However, the number of -686 G alleles was correlated with both neutrophil activity and mononuclear cell infiltration (p=0.036 and p=0.010, respectively), while the -650 C/C genotype was an independent risk factor for mononuclear cell infiltration (p=0.021 by ANOVA). In addition, both the number of -686 G alleles and the -650 C/C genotype showed an interaction with H. pylori infection to promote the infiltration of mononuclear cells (p=0.037 by ANCOVA and p=0.041 by ANOVA, respectively). Nrf2 promoter polymorphisms are significantly associated with the development of gastric mucosal inflammation, either independently or by interacting with H. pylori infection.

Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalInternational Journal of Molecular Medicine
Volume19
Issue number1
Publication statusPublished - 01-01-2007

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Helicobacter Infections
Gastritis
Helicobacter pylori
Alleles
Peptic Ulcer
Genes
Single Nucleotide Polymorphism
Stomach
Analysis of Variance
Genotype
Biopsy
Single-Stranded Conformational Polymorphism
Gastrointestinal Endoscopy
Endoscopy
Atrophy
Blood Cells
Neutrophils
Transcription Factors
Antioxidants
Inflammation

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Arisawa, Tomiyasu ; Tahara, Tomomitsu ; Shibata, Tomoyuki ; Nagasaka, Mitsuo ; Nakamura, Masakatsu ; Kamiya, Yoshio ; Fujita, Hiroshi ; Hasegawa, Shin ; Takagi, Tamaki ; Wang, Fang Yu ; Hirata, Ichiro ; Nakano, Hiroshi. / The relationship between Helicobacter pylori infection and promoter polymorphism of the Nrf2 gene in chronic gastritis. In: International Journal of Molecular Medicine. 2007 ; Vol. 19, No. 1. pp. 143-148.
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Arisawa, T, Tahara, T, Shibata, T, Nagasaka, M, Nakamura, M, Kamiya, Y, Fujita, H, Hasegawa, S, Takagi, T, Wang, FY, Hirata, I & Nakano, H 2007, 'The relationship between Helicobacter pylori infection and promoter polymorphism of the Nrf2 gene in chronic gastritis', International Journal of Molecular Medicine, vol. 19, no. 1, pp. 143-148.

The relationship between Helicobacter pylori infection and promoter polymorphism of the Nrf2 gene in chronic gastritis. / Arisawa, Tomiyasu; Tahara, Tomomitsu; Shibata, Tomoyuki; Nagasaka, Mitsuo; Nakamura, Masakatsu; Kamiya, Yoshio; Fujita, Hiroshi; Hasegawa, Shin; Takagi, Tamaki; Wang, Fang Yu; Hirata, Ichiro; Nakano, Hiroshi.

In: International Journal of Molecular Medicine, Vol. 19, No. 1, 01.01.2007, p. 143-148.

Research output: Contribution to journalArticle

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AU - Shibata, Tomoyuki

AU - Nagasaka, Mitsuo

AU - Nakamura, Masakatsu

AU - Kamiya, Yoshio

AU - Fujita, Hiroshi

AU - Hasegawa, Shin

AU - Takagi, Tamaki

AU - Wang, Fang Yu

AU - Hirata, Ichiro

AU - Nakano, Hiroshi

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