The relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies, Mn-SOD and carotenoids in rural Japanese residents

Koji Suzuki, Yoshinori Ito, Junichi Ochiai, Kunio Aoki, Kenji Wakai, Akiko Tamakoshi, Masahiko Ando, Yoshiyuki Watanabe, Kotaro Ozasa, Nao Seki, Yoshikazu Nishino, Takaaki Kondo, Yoshiyuki Ohno

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Abstract

This study investigated the relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies (oLAB), Mn-SOD, and carotenoids. Subjects were 79 males (mean age ± standard deviation; 62.1±10.0 years) and 79 females (60.3±10.3 y) who attended a health examination screening in the town of Hokkaido, Japan. Serum 8-OHdG, Mn-SOD, and oLAB levels were measured by ELISA and serum carotenoids levels were measured by HPLC. Smoking habits were assessed by public health nurses using a questionnaire. Serum 8-OHdG levels were significantly higher in males than in females. On the other hand, serum levels of β-carotene, α-carotene, β-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males than in females. Serum β-carotene, β-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males who were current smokers, compared to non-smokers. Serum 8-OHdG levels were also significantly higher in current smokers. Furthermore, in males, serum oLAB and β-carotene levels were significantly and negatively correlated with the number of cigarettes smoked per day. Serum Mn-SOD levels were unrelated to smoking habits in males. In conclusion, this preliminary study suggests that cigarette smoking increases serum 8-OHdG levels and reduces serum levels of oLAB and carotenoids, such as β-carotene, β-cryptoxanthin, and zeaxanthin and lutein in healthy male subjects. Serum levels of 8-OHdG, oLAB, and carotenoids may be useful biomarkers of oxidative conditions affected by smoking.

Original languageEnglish
Pages (from-to)29-37
Number of pages9
JournalJournal of epidemiology
Volume13
Issue number1
DOIs
Publication statusPublished - 01-01-2003

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Carotenoids
Superoxide Dismutase
Habits
Smoking
Antibodies
Serum
Lutein
oxidized low density lipoprotein
Public Health Nurses
Tobacco Products
Healthy Volunteers
Japan
Biomarkers
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Epidemiology

Cite this

Suzuki, Koji ; Ito, Yoshinori ; Ochiai, Junichi ; Aoki, Kunio ; Wakai, Kenji ; Tamakoshi, Akiko ; Ando, Masahiko ; Watanabe, Yoshiyuki ; Ozasa, Kotaro ; Seki, Nao ; Nishino, Yoshikazu ; Kondo, Takaaki ; Ohno, Yoshiyuki. / The relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies, Mn-SOD and carotenoids in rural Japanese residents. In: Journal of epidemiology. 2003 ; Vol. 13, No. 1. pp. 29-37.
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abstract = "This study investigated the relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies (oLAB), Mn-SOD, and carotenoids. Subjects were 79 males (mean age ± standard deviation; 62.1±10.0 years) and 79 females (60.3±10.3 y) who attended a health examination screening in the town of Hokkaido, Japan. Serum 8-OHdG, Mn-SOD, and oLAB levels were measured by ELISA and serum carotenoids levels were measured by HPLC. Smoking habits were assessed by public health nurses using a questionnaire. Serum 8-OHdG levels were significantly higher in males than in females. On the other hand, serum levels of β-carotene, α-carotene, β-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males than in females. Serum β-carotene, β-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males who were current smokers, compared to non-smokers. Serum 8-OHdG levels were also significantly higher in current smokers. Furthermore, in males, serum oLAB and β-carotene levels were significantly and negatively correlated with the number of cigarettes smoked per day. Serum Mn-SOD levels were unrelated to smoking habits in males. In conclusion, this preliminary study suggests that cigarette smoking increases serum 8-OHdG levels and reduces serum levels of oLAB and carotenoids, such as β-carotene, β-cryptoxanthin, and zeaxanthin and lutein in healthy male subjects. Serum levels of 8-OHdG, oLAB, and carotenoids may be useful biomarkers of oxidative conditions affected by smoking.",
author = "Koji Suzuki and Yoshinori Ito and Junichi Ochiai and Kunio Aoki and Kenji Wakai and Akiko Tamakoshi and Masahiko Ando and Yoshiyuki Watanabe and Kotaro Ozasa and Nao Seki and Yoshikazu Nishino and Takaaki Kondo and Yoshiyuki Ohno",
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Suzuki, K, Ito, Y, Ochiai, J, Aoki, K, Wakai, K, Tamakoshi, A, Ando, M, Watanabe, Y, Ozasa, K, Seki, N, Nishino, Y, Kondo, T & Ohno, Y 2003, 'The relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies, Mn-SOD and carotenoids in rural Japanese residents', Journal of epidemiology, vol. 13, no. 1, pp. 29-37. https://doi.org/10.1016/S1567-5688(03)90122-X

The relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies, Mn-SOD and carotenoids in rural Japanese residents. / Suzuki, Koji; Ito, Yoshinori; Ochiai, Junichi; Aoki, Kunio; Wakai, Kenji; Tamakoshi, Akiko; Ando, Masahiko; Watanabe, Yoshiyuki; Ozasa, Kotaro; Seki, Nao; Nishino, Yoshikazu; Kondo, Takaaki; Ohno, Yoshiyuki.

In: Journal of epidemiology, Vol. 13, No. 1, 01.01.2003, p. 29-37.

Research output: Contribution to journalArticle

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T1 - The relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies, Mn-SOD and carotenoids in rural Japanese residents

AU - Suzuki, Koji

AU - Ito, Yoshinori

AU - Ochiai, Junichi

AU - Aoki, Kunio

AU - Wakai, Kenji

AU - Tamakoshi, Akiko

AU - Ando, Masahiko

AU - Watanabe, Yoshiyuki

AU - Ozasa, Kotaro

AU - Seki, Nao

AU - Nishino, Yoshikazu

AU - Kondo, Takaaki

AU - Ohno, Yoshiyuki

PY - 2003/1/1

Y1 - 2003/1/1

N2 - This study investigated the relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies (oLAB), Mn-SOD, and carotenoids. Subjects were 79 males (mean age ± standard deviation; 62.1±10.0 years) and 79 females (60.3±10.3 y) who attended a health examination screening in the town of Hokkaido, Japan. Serum 8-OHdG, Mn-SOD, and oLAB levels were measured by ELISA and serum carotenoids levels were measured by HPLC. Smoking habits were assessed by public health nurses using a questionnaire. Serum 8-OHdG levels were significantly higher in males than in females. On the other hand, serum levels of β-carotene, α-carotene, β-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males than in females. Serum β-carotene, β-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males who were current smokers, compared to non-smokers. Serum 8-OHdG levels were also significantly higher in current smokers. Furthermore, in males, serum oLAB and β-carotene levels were significantly and negatively correlated with the number of cigarettes smoked per day. Serum Mn-SOD levels were unrelated to smoking habits in males. In conclusion, this preliminary study suggests that cigarette smoking increases serum 8-OHdG levels and reduces serum levels of oLAB and carotenoids, such as β-carotene, β-cryptoxanthin, and zeaxanthin and lutein in healthy male subjects. Serum levels of 8-OHdG, oLAB, and carotenoids may be useful biomarkers of oxidative conditions affected by smoking.

AB - This study investigated the relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies (oLAB), Mn-SOD, and carotenoids. Subjects were 79 males (mean age ± standard deviation; 62.1±10.0 years) and 79 females (60.3±10.3 y) who attended a health examination screening in the town of Hokkaido, Japan. Serum 8-OHdG, Mn-SOD, and oLAB levels were measured by ELISA and serum carotenoids levels were measured by HPLC. Smoking habits were assessed by public health nurses using a questionnaire. Serum 8-OHdG levels were significantly higher in males than in females. On the other hand, serum levels of β-carotene, α-carotene, β-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males than in females. Serum β-carotene, β-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males who were current smokers, compared to non-smokers. Serum 8-OHdG levels were also significantly higher in current smokers. Furthermore, in males, serum oLAB and β-carotene levels were significantly and negatively correlated with the number of cigarettes smoked per day. Serum Mn-SOD levels were unrelated to smoking habits in males. In conclusion, this preliminary study suggests that cigarette smoking increases serum 8-OHdG levels and reduces serum levels of oLAB and carotenoids, such as β-carotene, β-cryptoxanthin, and zeaxanthin and lutein in healthy male subjects. Serum levels of 8-OHdG, oLAB, and carotenoids may be useful biomarkers of oxidative conditions affected by smoking.

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