The role of the tumor necrosis factor receptor in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice

Minoru Nakai, Kaori Sudo, Yasuhiro Yamada, Yasushi Kojima, Tomohiro Kato, Kuniaki Saito, Hisataka Moriwaki, Mitsuru Seishima

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22 Citations (Scopus)

Abstract

We investigated the effect of TNBS administration using TNF-receptor knockout mice to elucidate the role of TNF receptors in chronic inflammation of the colon. Histologically, inflammatory cell scores showed no significant differences among TNBS-administered groups, while tissue damage scores were significantly lower in TNFR-1KO and TNFR-1,2KO than in WT. The apoptotic indexes of lamina propria mononuclear cells (LPMC) of all TNBS-administered groups were significantly lower than that of controls. TNF-α mRNA expression in the colon was significantly higher in all TNBS-administered groups than in controls. And NF-κ B activities were enhanced in WT and TNFR-2KO compared with controls. Our data indicate that the TNF/TNFR-1 signaling system mediates mucosal damage through the enhancement of NF-κ B activity and that continuous infiltration of TNF-producing cells, probably a key pathogeneses of colitis, may be closely associated with defective apoptosis of LPMC, which is possibly independent of the TNF/TNFR signaling system in TNBS-induced colitis.

Original languageEnglish
Pages (from-to)1669-1676
Number of pages8
JournalDigestive Diseases and Sciences
Volume50
Issue number9
DOIs
Publication statusPublished - 01-09-2005

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All Science Journal Classification (ASJC) codes

  • Physiology
  • Gastroenterology

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