The role of the tumor necrosis factor receptor in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice

Minoru Nakai, Kaori Sudo, Yasuhiro Yamada, Yasushi Kojima, Tomohiro Kato, Kuniaki Saito, Hisataka Moriwaki, Mitsuru Seishima

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We investigated the effect of TNBS administration using TNF-receptor knockout mice to elucidate the role of TNF receptors in chronic inflammation of the colon. Histologically, inflammatory cell scores showed no significant differences among TNBS-administered groups, while tissue damage scores were significantly lower in TNFR-1KO and TNFR-1,2KO than in WT. The apoptotic indexes of lamina propria mononuclear cells (LPMC) of all TNBS-administered groups were significantly lower than that of controls. TNF-α mRNA expression in the colon was significantly higher in all TNBS-administered groups than in controls. And NF-κ B activities were enhanced in WT and TNFR-2KO compared with controls. Our data indicate that the TNF/TNFR-1 signaling system mediates mucosal damage through the enhancement of NF-κ B activity and that continuous infiltration of TNF-producing cells, probably a key pathogeneses of colitis, may be closely associated with defective apoptosis of LPMC, which is possibly independent of the TNF/TNFR signaling system in TNBS-induced colitis.

Original languageEnglish
Pages (from-to)1669-1676
Number of pages8
JournalDigestive Diseases and Sciences
Volume50
Issue number9
DOIs
Publication statusPublished - 01-09-2005
Externally publishedYes

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Trinitrobenzenes
Receptors, Tumor Necrosis Factor, Type II
Sulfonic Acids
Colitis
Tumor Necrosis Factor Receptors
Colon
Mucous Membrane
Knockout Mice
sym-trinitrobenzene
Apoptosis
Inflammation
Control Groups
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Physiology
  • Gastroenterology

Cite this

Nakai, Minoru ; Sudo, Kaori ; Yamada, Yasuhiro ; Kojima, Yasushi ; Kato, Tomohiro ; Saito, Kuniaki ; Moriwaki, Hisataka ; Seishima, Mitsuru. / The role of the tumor necrosis factor receptor in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. In: Digestive Diseases and Sciences. 2005 ; Vol. 50, No. 9. pp. 1669-1676.
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The role of the tumor necrosis factor receptor in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. / Nakai, Minoru; Sudo, Kaori; Yamada, Yasuhiro; Kojima, Yasushi; Kato, Tomohiro; Saito, Kuniaki; Moriwaki, Hisataka; Seishima, Mitsuru.

In: Digestive Diseases and Sciences, Vol. 50, No. 9, 01.09.2005, p. 1669-1676.

Research output: Contribution to journalArticle

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AU - Nakai, Minoru

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AU - Kato, Tomohiro

AU - Saito, Kuniaki

AU - Moriwaki, Hisataka

AU - Seishima, Mitsuru

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