The Sac3 homologue shd1 is involved in mitotic progression in mammalian cells

Sefat-E-Khuda, Mikoto Yoshida, Yan Xing, Tatsuya Shimasaki, Motohiro Takeya, Kazuhiko Kuwahara, Nobuo Sakaguchi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Saccharomyces Sac3 required for actin assembly was shown to be involved in DNA replication. Here, we studied the function of a mammalian homologue SHD1 in cell cycle progression. SHD1 is localized on centrosomes at interphase and at spindle poles and mitotic spindles, similar to α-tubulin, at M phase. RNA interference suppression of endogenous shd1 caused defects in centresome duplication and spindle formation displaying cells with a single apparent centrosome and down-regulated Mad2 expression, generating increased micronuclei. Conversely, increased expression of SHD1 by DNA transfection with shd1-green fluorescent protein (gfp) vector for a fusion protein of SHD1 and GFP caused abnormalities in centrosome duplication displaying cells with multiple centrosomes and deregulated spindle assembly with up-regulated Mad2 expression until anaphase, generating polyploidy cells. These results demonstrated that shd1 is involved in cell cycle progression, in particular centrosome duplication and a spindle assembly checkpoint function.

Original languageEnglish
Pages (from-to)46182-46190
Number of pages9
JournalJournal of Biological Chemistry
Volume279
Issue number44
DOIs
Publication statusPublished - 29-10-2004

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Sefat-E-Khuda, Yoshida, M., Xing, Y., Shimasaki, T., Takeya, M., Kuwahara, K., & Sakaguchi, N. (2004). The Sac3 homologue shd1 is involved in mitotic progression in mammalian cells. Journal of Biological Chemistry, 279(44), 46182-46190. https://doi.org/10.1074/jbc.M405347200