The signaling adaptor protein CD3ζ is a negative regulator of dendrite development in young neurons

Stéphane J. Baudouin, Julie Angibaud, Gildas Loussouarn, Virginie Bonnamain, Akihiro Matsuura, Miyuki Kinebuchi, Philippe Naveilhan, Hélène Boudin

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A novel idea is emerging that a large molecular repertoire is common to the nervous and immune systems, which might reflect the existence of novel neuronal functions for immune molecules in the brain. Here, we show that the transmembrane adaptor signaling protein CD3ζ, first described in the immune system, has a previously uncharacterized role in regulating neuronal development. Biochemical and immunohistochemical analyses of the rat brain and cultured neurons showed that CD3ζ is mainly expressed in neurons. Distribution of CD3ζ in developing cultured hippocampal neurons, as determined by immunofluorescence, indicates that CD3ζ is preferentially associated with the somatodendritic compartment as soon as the dendrites initiate their differentiation. At this stage, CD3ζ was selectively concentrated at dendritic filopodia and growth cones, actin-rich structures involved in neurite growth and patterning. siRNA-mediated knockdown of CD3ζ in cultured neurons or overexpression of a loss-of-function CD3ζ mutant lacking the tyrosine phosphorylation sites in the immunoreceptor tyrosine-based activation motifs (ITAMs) increased dendritic arborization. Conversely, activation of endogenous CD3ζ by a CD3ζ antibody reduced the size of the dendritic arbor. Altogether, our findings reveal a novel role for CD3ζ in the nervous system, suggesting its contribution to dendrite development through ITAM-based mechanisms.

Original languageEnglish
Pages (from-to)2444-2456
Number of pages13
JournalMolecular Biology of the Cell
Volume19
Issue number6
DOIs
Publication statusPublished - 01-06-2008

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Dendrites
Immunoreceptor Tyrosine-Based Activation Motif
Neurons
Nervous System
Immune System
Proteins
Growth Cones
Pseudopodia
Neuronal Plasticity
Brain
Neurites
Small Interfering RNA
Fluorescent Antibody Technique
Tyrosine
Actins
Phosphorylation
Antibodies
Growth

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Baudouin, S. J., Angibaud, J., Loussouarn, G., Bonnamain, V., Matsuura, A., Kinebuchi, M., ... Boudin, H. (2008). The signaling adaptor protein CD3ζ is a negative regulator of dendrite development in young neurons. Molecular Biology of the Cell, 19(6), 2444-2456. https://doi.org/10.1091/mbc.E07-09-0947
Baudouin, Stéphane J. ; Angibaud, Julie ; Loussouarn, Gildas ; Bonnamain, Virginie ; Matsuura, Akihiro ; Kinebuchi, Miyuki ; Naveilhan, Philippe ; Boudin, Hélène. / The signaling adaptor protein CD3ζ is a negative regulator of dendrite development in young neurons. In: Molecular Biology of the Cell. 2008 ; Vol. 19, No. 6. pp. 2444-2456.
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Baudouin, SJ, Angibaud, J, Loussouarn, G, Bonnamain, V, Matsuura, A, Kinebuchi, M, Naveilhan, P & Boudin, H 2008, 'The signaling adaptor protein CD3ζ is a negative regulator of dendrite development in young neurons', Molecular Biology of the Cell, vol. 19, no. 6, pp. 2444-2456. https://doi.org/10.1091/mbc.E07-09-0947

The signaling adaptor protein CD3ζ is a negative regulator of dendrite development in young neurons. / Baudouin, Stéphane J.; Angibaud, Julie; Loussouarn, Gildas; Bonnamain, Virginie; Matsuura, Akihiro; Kinebuchi, Miyuki; Naveilhan, Philippe; Boudin, Hélène.

In: Molecular Biology of the Cell, Vol. 19, No. 6, 01.06.2008, p. 2444-2456.

Research output: Contribution to journalArticle

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