The simple in vitro thrombogenic test: Modified methods for same priming pumps

Eiki Tayama, Satoshi Ohtsubo, Tadashi Nakazawa, Yoshiyuki Takami, Yoshinari Niimi, Kenzo Makinouchi, Julia A. Glueck, Yukihiko Nosé

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5 Citations (Scopus)


The improvement of antithrombogenicity is one of the major objectives for the development of blood pumps. Previously we reported that an in vitro thrombogenic test was useful as a pilot study, especially to predict thrombogenic areas. In this study we modified the method for testing pumps with identical priming volumes by eliminating the blood reservoir. Identical compact mock loops (priming volume of 53 ml, without pump) were constructed and tested with the same priming volume of Nikkiso centrifugal pumps, noncoated verus heparincoated. Two pumps were run simultaneously using the same source of fresh heparinized human blood (activated clotting time [ACT] 150-250 s) for 4 or 6 h. Results indicated that the heparin-coated pump had a longer thrombus free period than the noncoated one. The thrombi location and forms were consistently in the same places the in vivo study had identified. It is suggested that this modified in vitro thrombogenic test is a feasible pilot study, as well as the one previously reported. The minimal priming volume will allow evaluation of multiple pumps simultaneously with the same source blood.

Original languageEnglish
Pages (from-to)1305-1308
Number of pages4
JournalArtificial Organs
Issue number12
Publication statusPublished - 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering


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