TY - JOUR
T1 - The smg GDS-induced activation of smg p21 is initiated by cyclic AMP-dependent protein kinase-catalyzed phosphorylation of smg p21
AU - Itoh, Takahito
AU - Kaibuchi, Kozo
AU - Sasaki, Takuya
AU - Takai, Yoshimi
PY - 1991/6/28
Y1 - 1991/6/28
N2 - We have shown that smg p21B is phosphorylated by cyclic AMP-dependent protein kinase (protein kinase A) and that membrane acidic phospholipids such as phosphatidic acid and phosphatidylinositol markedly inhibit the smg GDS-induced activation of smg p21B. However, we show here that phosphatidic acid and phosphatidylinositol exhibit a less inhibitory effect on the smg GDS-induced activation of the phosphorylated form of smg p21B. Thus, in the presence of membrane acidic phospholipids, the smg GDS-induced activation of smg p21B is totally dependent on the protein kinase A-catalyzed phosphorylation of smg p21B. Since smg p21B is located on the membranes in resting cells, it is likely that the smg GDS-induced activation of smg p21B is initiated by the protein kinase A activation.
AB - We have shown that smg p21B is phosphorylated by cyclic AMP-dependent protein kinase (protein kinase A) and that membrane acidic phospholipids such as phosphatidic acid and phosphatidylinositol markedly inhibit the smg GDS-induced activation of smg p21B. However, we show here that phosphatidic acid and phosphatidylinositol exhibit a less inhibitory effect on the smg GDS-induced activation of the phosphorylated form of smg p21B. Thus, in the presence of membrane acidic phospholipids, the smg GDS-induced activation of smg p21B is totally dependent on the protein kinase A-catalyzed phosphorylation of smg p21B. Since smg p21B is located on the membranes in resting cells, it is likely that the smg GDS-induced activation of smg p21B is initiated by the protein kinase A activation.
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U2 - 10.1016/0006-291X(91)90685-Z
DO - 10.1016/0006-291X(91)90685-Z
M3 - Article
C2 - 1905539
AN - SCOPUS:0025895931
VL - 177
SP - 1319
EP - 1324
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -