TY - JOUR
T1 - The smg GDS-induced activation of smg p21 is initiated by cyclic AMP-dependent protein kinase-catalyzed phosphorylation of smg p21
AU - Itoh, Takahito
AU - Kaibuchi, Kozo
AU - Sasaki, Takuya
AU - Takai, Yoshimi
N1 - Funding Information:
The smg p21 family, composed of A and B members, belongs to a z-as p2l/ras @l-like small G protein superfamily (l-5, for reviews, see Refs. 6-8). smg ~21s have the same putative effector domain as that of ras ~21s and could exert the actions similar or antagonistic to those of ras ~21s (l-5). smg ~21s have the same C- terminal motif of Cys-A-A-X (A: aliphatic amino acid; X: any amino acid) as that of ras ~21s which is post-translationally processed 'This investigation was supported by grants-in-aid Scientific Research and Cancer Research from the Ministry Education, Science, and Culture, Japan (1991), a grant-in-aid for Abnormalities in Hormone Receptor Mechanisms from the Ministry of Health and Welfare, Japan (1991), and by grants from the Human Frontier Science Program (1990), the Yamanouchi Foundation for Research on Metabolic Disease (1990), and the Research Program on Cell Calcium Signal in the Cardiovascular System (1990), Japan.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1991/6/28
Y1 - 1991/6/28
N2 - We have shown that smg p21B is phosphorylated by cyclic AMP-dependent protein kinase (protein kinase A) and that membrane acidic phospholipids such as phosphatidic acid and phosphatidylinositol markedly inhibit the smg GDS-induced activation of smg p21B. However, we show here that phosphatidic acid and phosphatidylinositol exhibit a less inhibitory effect on the smg GDS-induced activation of the phosphorylated form of smg p21B. Thus, in the presence of membrane acidic phospholipids, the smg GDS-induced activation of smg p21B is totally dependent on the protein kinase A-catalyzed phosphorylation of smg p21B. Since smg p21B is located on the membranes in resting cells, it is likely that the smg GDS-induced activation of smg p21B is initiated by the protein kinase A activation.
AB - We have shown that smg p21B is phosphorylated by cyclic AMP-dependent protein kinase (protein kinase A) and that membrane acidic phospholipids such as phosphatidic acid and phosphatidylinositol markedly inhibit the smg GDS-induced activation of smg p21B. However, we show here that phosphatidic acid and phosphatidylinositol exhibit a less inhibitory effect on the smg GDS-induced activation of the phosphorylated form of smg p21B. Thus, in the presence of membrane acidic phospholipids, the smg GDS-induced activation of smg p21B is totally dependent on the protein kinase A-catalyzed phosphorylation of smg p21B. Since smg p21B is located on the membranes in resting cells, it is likely that the smg GDS-induced activation of smg p21B is initiated by the protein kinase A activation.
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U2 - 10.1016/0006-291X(91)90685-Z
DO - 10.1016/0006-291X(91)90685-Z
M3 - Article
C2 - 1905539
AN - SCOPUS:0025895931
VL - 177
SP - 1319
EP - 1324
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -