The therapeutic effects of the herbal medicine, Juzen-taiho-to, on amyloid-β burden in a mouse model of alzheimer's disease

Hideo Hara, Seiko Kataoka, Mayumi Anan, Akihiro Ueda, Tatsuro Mutoh, Takeshi Tabira

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Innate immunity, especially that involving macrophage function, reportedly diminishes with advancing age and in patients with Alzheimer's disease (AD). In this study, we tried to elicit the non-specific activation of peripheral macrophages by oral administration of the herbal medicine Juzen-taiho-to (JTT), to assess its effect as a possible treatment for AD patients. Amyloid-β protein precursor transgenic mice were used as a model of AD to clarify the effect of JTT. Activated macrophages derived from bone marrow cross the blood-brain barrier, and then develop into microglia, which phagocytose aggregated amyloid-β (Aβ) in senile plaques. Here we show that orally administered JTT increased the number of CD11b-positive ramified microglia in the mouse brain. The immunohistochemical examination of brain sections stained with polyclonal anti-Aβ antibody showed reduced Aβ burden, and Aβ levels were also decreased in the insoluble fractions of brain homogenates, as determined by ELISA. Thus, the activation of peripheral macrophages by JTT might be a potential new therapeutic strategy for AD.

Original languageEnglish
Pages (from-to)427-439
Number of pages13
JournalJournal of Alzheimer's Disease
Volume20
Issue number2
DOIs
Publication statusPublished - 01-01-2010

Fingerprint

Herbal Medicine
Therapeutic Uses
Amyloid
Alzheimer Disease
Macrophage Activation
Microglia
Brain
Macrophages
Amyloid beta-Protein Precursor
Amyloid Plaques
Blood-Brain Barrier
Phagocytosis
Innate Immunity
Transgenic Mice
Oral Administration
Anti-Idiotypic Antibodies
Enzyme-Linked Immunosorbent Assay
juzentaihoto
Therapeutics

All Science Journal Classification (ASJC) codes

  • Clinical Psychology
  • Geriatrics and Gerontology
  • Psychiatry and Mental health

Cite this

Hara, Hideo ; Kataoka, Seiko ; Anan, Mayumi ; Ueda, Akihiro ; Mutoh, Tatsuro ; Tabira, Takeshi. / The therapeutic effects of the herbal medicine, Juzen-taiho-to, on amyloid-β burden in a mouse model of alzheimer's disease. In: Journal of Alzheimer's Disease. 2010 ; Vol. 20, No. 2. pp. 427-439.
@article{7a4befb3f0634f7da9ebe2131c31948d,
title = "The therapeutic effects of the herbal medicine, Juzen-taiho-to, on amyloid-β burden in a mouse model of alzheimer's disease",
abstract = "Innate immunity, especially that involving macrophage function, reportedly diminishes with advancing age and in patients with Alzheimer's disease (AD). In this study, we tried to elicit the non-specific activation of peripheral macrophages by oral administration of the herbal medicine Juzen-taiho-to (JTT), to assess its effect as a possible treatment for AD patients. Amyloid-β protein precursor transgenic mice were used as a model of AD to clarify the effect of JTT. Activated macrophages derived from bone marrow cross the blood-brain barrier, and then develop into microglia, which phagocytose aggregated amyloid-β (Aβ) in senile plaques. Here we show that orally administered JTT increased the number of CD11b-positive ramified microglia in the mouse brain. The immunohistochemical examination of brain sections stained with polyclonal anti-Aβ antibody showed reduced Aβ burden, and Aβ levels were also decreased in the insoluble fractions of brain homogenates, as determined by ELISA. Thus, the activation of peripheral macrophages by JTT might be a potential new therapeutic strategy for AD.",
author = "Hideo Hara and Seiko Kataoka and Mayumi Anan and Akihiro Ueda and Tatsuro Mutoh and Takeshi Tabira",
year = "2010",
month = "1",
day = "1",
doi = "10.3233/JAD-2010-1381",
language = "English",
volume = "20",
pages = "427--439",
journal = "Journal of Alzheimer's Disease",
issn = "1387-2877",
publisher = "IOS Press",
number = "2",

}

The therapeutic effects of the herbal medicine, Juzen-taiho-to, on amyloid-β burden in a mouse model of alzheimer's disease. / Hara, Hideo; Kataoka, Seiko; Anan, Mayumi; Ueda, Akihiro; Mutoh, Tatsuro; Tabira, Takeshi.

In: Journal of Alzheimer's Disease, Vol. 20, No. 2, 01.01.2010, p. 427-439.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The therapeutic effects of the herbal medicine, Juzen-taiho-to, on amyloid-β burden in a mouse model of alzheimer's disease

AU - Hara, Hideo

AU - Kataoka, Seiko

AU - Anan, Mayumi

AU - Ueda, Akihiro

AU - Mutoh, Tatsuro

AU - Tabira, Takeshi

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Innate immunity, especially that involving macrophage function, reportedly diminishes with advancing age and in patients with Alzheimer's disease (AD). In this study, we tried to elicit the non-specific activation of peripheral macrophages by oral administration of the herbal medicine Juzen-taiho-to (JTT), to assess its effect as a possible treatment for AD patients. Amyloid-β protein precursor transgenic mice were used as a model of AD to clarify the effect of JTT. Activated macrophages derived from bone marrow cross the blood-brain barrier, and then develop into microglia, which phagocytose aggregated amyloid-β (Aβ) in senile plaques. Here we show that orally administered JTT increased the number of CD11b-positive ramified microglia in the mouse brain. The immunohistochemical examination of brain sections stained with polyclonal anti-Aβ antibody showed reduced Aβ burden, and Aβ levels were also decreased in the insoluble fractions of brain homogenates, as determined by ELISA. Thus, the activation of peripheral macrophages by JTT might be a potential new therapeutic strategy for AD.

AB - Innate immunity, especially that involving macrophage function, reportedly diminishes with advancing age and in patients with Alzheimer's disease (AD). In this study, we tried to elicit the non-specific activation of peripheral macrophages by oral administration of the herbal medicine Juzen-taiho-to (JTT), to assess its effect as a possible treatment for AD patients. Amyloid-β protein precursor transgenic mice were used as a model of AD to clarify the effect of JTT. Activated macrophages derived from bone marrow cross the blood-brain barrier, and then develop into microglia, which phagocytose aggregated amyloid-β (Aβ) in senile plaques. Here we show that orally administered JTT increased the number of CD11b-positive ramified microglia in the mouse brain. The immunohistochemical examination of brain sections stained with polyclonal anti-Aβ antibody showed reduced Aβ burden, and Aβ levels were also decreased in the insoluble fractions of brain homogenates, as determined by ELISA. Thus, the activation of peripheral macrophages by JTT might be a potential new therapeutic strategy for AD.

UR - http://www.scopus.com/inward/record.url?scp=77954491919&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954491919&partnerID=8YFLogxK

U2 - 10.3233/JAD-2010-1381

DO - 10.3233/JAD-2010-1381

M3 - Article

C2 - 20164567

AN - SCOPUS:77954491919

VL - 20

SP - 427

EP - 439

JO - Journal of Alzheimer's Disease

JF - Journal of Alzheimer's Disease

SN - 1387-2877

IS - 2

ER -