The US11 gene product of herpes simplex virus has intercellular trafficking activity

Tetsuo Koshizuka, Hiroki Takakuwa, Fumi Goshima, Takayuki Murata, Yukihiro Nishiyama

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11 Citations (Scopus)

Abstract

The US11 gene product of herpes simplex virus is an abundant virion structural protein with RNA-binding regulatory activity. Its carboxyl-terminal half consists of tandem tripeptide repeats of the sequence RXP. We demonstrate that the US11 protein has intercellular trafficking activity and accumulates in the nucleolus when singly expressed in cultured cells, and that the RXP repeats are responsible for this activity. These same properties were also observed in cells expressing a fusion protein linking US11 to the green fluorescent protein. Furthermore, exogenous US11 protein was internalized by cells at 4°C, which suggests that US11 protein uptake occurs primarily through an energy-independent pathway.

Original languageEnglish
Pages (from-to)597-602
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume288
Issue number3
DOIs
Publication statusPublished - 02-11-2001

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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