TY - JOUR
T1 - The US11 gene product of herpes simplex virus has intercellular trafficking activity
AU - Koshizuka, Tetsuo
AU - Takakuwa, Hiroki
AU - Goshima, Fumi
AU - Murata, Takayuki
AU - Nishiyama, Yukihiro
N1 - Funding Information:
We thank E. Iwata and T. Tsuruguchi for their technical assistance. This work was supported by a grant from the Japan Society for the Promotion of Science (JSPS-RFTF97L00703) and also by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan.
PY - 2001/11/2
Y1 - 2001/11/2
N2 - The US11 gene product of herpes simplex virus is an abundant virion structural protein with RNA-binding regulatory activity. Its carboxyl-terminal half consists of tandem tripeptide repeats of the sequence RXP. We demonstrate that the US11 protein has intercellular trafficking activity and accumulates in the nucleolus when singly expressed in cultured cells, and that the RXP repeats are responsible for this activity. These same properties were also observed in cells expressing a fusion protein linking US11 to the green fluorescent protein. Furthermore, exogenous US11 protein was internalized by cells at 4°C, which suggests that US11 protein uptake occurs primarily through an energy-independent pathway.
AB - The US11 gene product of herpes simplex virus is an abundant virion structural protein with RNA-binding regulatory activity. Its carboxyl-terminal half consists of tandem tripeptide repeats of the sequence RXP. We demonstrate that the US11 protein has intercellular trafficking activity and accumulates in the nucleolus when singly expressed in cultured cells, and that the RXP repeats are responsible for this activity. These same properties were also observed in cells expressing a fusion protein linking US11 to the green fluorescent protein. Furthermore, exogenous US11 protein was internalized by cells at 4°C, which suggests that US11 protein uptake occurs primarily through an energy-independent pathway.
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U2 - 10.1006/bbrc.2001.5790
DO - 10.1006/bbrc.2001.5790
M3 - Article
C2 - 11676485
AN - SCOPUS:0035798251
SN - 0006-291X
VL - 288
SP - 597
EP - 602
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -