The Xenopus Bowline/Ripply family proteins negatively regulate the transcriptional activity of T-box transcription factors

Keisuke Hitachi, Hiroki Danno, Shunsuke Tazumi, Yuko Aihara, Hideho Uchiyama, Koji Okabayashi, Akiko Kondo, Makoto Asashima

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Bowline, which is a member of the Xenopus Bowline/Ripply family of proteins, represses the transcription of somitogenesis-related genes before somite segmentation, which makes Bowline indispensable for somitogenesis. Although there are three bowline/Ripply family genes in each vertebrate species, it is not known whether the Bowline/Ripply family proteins share a common role in development. To elucidate their developmental roles, we examined the expression patterns and functions of the Xenopus Bowline/Ripply family proteins Bowline, Ledgerline, and a novel member of this protein family, xRipply3. We found that the expression patterns of bowline and ledgerline overlapped in the presomitic mesoderm (PSM), whereas ledgerline was additionally expressed in the newly formed somites. In addition, we isolated xRipply3, which is expressed in the pharyngeal region. Co-immunoprecipitation assays revealed that Ledgerline and xRipply3 interacted with T-box proteins and the transcriptional co-repressor Groucho/TLE. In luciferase assays, xRipply3 weakly suppressed the transcriptional activity of Tbx1, while Ledgerline strongly suppressed that of Tbx6. In line with the repressive role of Ledgerline, knockdown of Ledgerline resulted in enlargement of expression regions of the somitogenesis-related-genes mespb and Tbx6. Inhibition of histone deacetylase activity increased the expression of mespb, as seen in the Bowline and Ledgerline knockdown experiments. These results suggest that the Groucho-HDAC complex is required for the repressive activity of Bowline/Ripply family proteins during Xenopus somitogenesis. We conclude that although the Xenopus Bowline/Ripply family proteins Bowline, Ledgerline and xRipply3 are expressed differentially, they all act as negative regulators of T-box proteins.

Original languageEnglish
Pages (from-to)631-639
Number of pages9
JournalInternational Journal of Developmental Biology
Volume53
Issue number4
DOIs
Publication statusPublished - 01-12-2009

Fingerprint

Xenopus
Transcription Factors
Somites
Proteins
Xenopus Proteins
Genes
Co-Repressor Proteins
Histone Deacetylases
Mesoderm
Luciferases
Immunoprecipitation
Vertebrates

All Science Journal Classification (ASJC) codes

  • Embryology
  • Developmental Biology

Cite this

Hitachi, Keisuke ; Danno, Hiroki ; Tazumi, Shunsuke ; Aihara, Yuko ; Uchiyama, Hideho ; Okabayashi, Koji ; Kondo, Akiko ; Asashima, Makoto. / The Xenopus Bowline/Ripply family proteins negatively regulate the transcriptional activity of T-box transcription factors. In: International Journal of Developmental Biology. 2009 ; Vol. 53, No. 4. pp. 631-639.
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The Xenopus Bowline/Ripply family proteins negatively regulate the transcriptional activity of T-box transcription factors. / Hitachi, Keisuke; Danno, Hiroki; Tazumi, Shunsuke; Aihara, Yuko; Uchiyama, Hideho; Okabayashi, Koji; Kondo, Akiko; Asashima, Makoto.

In: International Journal of Developmental Biology, Vol. 53, No. 4, 01.12.2009, p. 631-639.

Research output: Contribution to journalArticle

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AU - Hitachi, Keisuke

AU - Danno, Hiroki

AU - Tazumi, Shunsuke

AU - Aihara, Yuko

AU - Uchiyama, Hideho

AU - Okabayashi, Koji

AU - Kondo, Akiko

AU - Asashima, Makoto

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