Abstract
Theanine (γ-glutamylethylamide), an amino acid in tea, is a putative neuroprotective and antioxidant compound capable of improving lifespan and cognitive function. Because we previously reported cognitive dysfunction in klotho mutant mice via down-regulation of janus kinase 2 (JAK2) and signal transducer and activator of transcription3 (STAT3), M1 muscarinic cholinergic receptor (M1 mAChR), and ERK signaling, we, therefore, investigated whether self-administration of theanine affects memory dysfunction in response to klotho gene depletion in mice, and whether theanine modulates the JAK2/STAT3, M1 mAChR, and ERK signaling network. Theanine significantly attenuated memory impairments in klotho mutant mice. Moreover, theanine self-administration significantly attenuated inhibitions of JAK2/STAT3 phosphorylation, M1 mAChR expression, and ERK 1/2 phosphorylation in the hippocampus of klotho mutant mice. Consistently, AG490, a JAK2/STAT3 inhibitor, dicyclomine, an M1 mAChR antagonist, or U0126, an ERK 1/2 inhibitor, significantly counteracted theanine-induced attenuation of memory impairment induced by klotho gene depletion in mice. Our study suggests that theanine attenuates memory impairments in a genetic aging model via up-regulation of JAK2/STAT3, M1 mAChR, and ERK signaling.
| Original language | English |
|---|---|
| Pages (from-to) | 325-332 |
| Number of pages | 8 |
| Journal | Food and Function |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 01-2019 |
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All Science Journal Classification (ASJC) codes
- Food Science
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Theanine attenuates memory impairments induced by : Klotho gene depletion in mice. / Nguyen, Bao Trong; Sharma, Naveen; Shin, Eun Joo; Jeong, Ji Hoon; Lee, Sung Hoon; Jang, Choon Gon; Nah, Seung Yeol; Nabeshima, Toshitaka; Yoneda, Yukio; Kim, Hyoung Chun.
In: Food and Function, Vol. 10, No. 1, 01.2019, p. 325-332.Research output: Contribution to journal › Article
TY - JOUR
T1 - Theanine attenuates memory impairments induced by
T2 - Klotho gene depletion in mice
AU - Nguyen, Bao Trong
AU - Sharma, Naveen
AU - Shin, Eun Joo
AU - Jeong, Ji Hoon
AU - Lee, Sung Hoon
AU - Jang, Choon Gon
AU - Nah, Seung Yeol
AU - Nabeshima, Toshitaka
AU - Yoneda, Yukio
AU - Kim, Hyoung Chun
PY - 2019/1
Y1 - 2019/1
N2 - Theanine (γ-glutamylethylamide), an amino acid in tea, is a putative neuroprotective and antioxidant compound capable of improving lifespan and cognitive function. Because we previously reported cognitive dysfunction in klotho mutant mice via down-regulation of janus kinase 2 (JAK2) and signal transducer and activator of transcription3 (STAT3), M1 muscarinic cholinergic receptor (M1 mAChR), and ERK signaling, we, therefore, investigated whether self-administration of theanine affects memory dysfunction in response to klotho gene depletion in mice, and whether theanine modulates the JAK2/STAT3, M1 mAChR, and ERK signaling network. Theanine significantly attenuated memory impairments in klotho mutant mice. Moreover, theanine self-administration significantly attenuated inhibitions of JAK2/STAT3 phosphorylation, M1 mAChR expression, and ERK 1/2 phosphorylation in the hippocampus of klotho mutant mice. Consistently, AG490, a JAK2/STAT3 inhibitor, dicyclomine, an M1 mAChR antagonist, or U0126, an ERK 1/2 inhibitor, significantly counteracted theanine-induced attenuation of memory impairment induced by klotho gene depletion in mice. Our study suggests that theanine attenuates memory impairments in a genetic aging model via up-regulation of JAK2/STAT3, M1 mAChR, and ERK signaling.
AB - Theanine (γ-glutamylethylamide), an amino acid in tea, is a putative neuroprotective and antioxidant compound capable of improving lifespan and cognitive function. Because we previously reported cognitive dysfunction in klotho mutant mice via down-regulation of janus kinase 2 (JAK2) and signal transducer and activator of transcription3 (STAT3), M1 muscarinic cholinergic receptor (M1 mAChR), and ERK signaling, we, therefore, investigated whether self-administration of theanine affects memory dysfunction in response to klotho gene depletion in mice, and whether theanine modulates the JAK2/STAT3, M1 mAChR, and ERK signaling network. Theanine significantly attenuated memory impairments in klotho mutant mice. Moreover, theanine self-administration significantly attenuated inhibitions of JAK2/STAT3 phosphorylation, M1 mAChR expression, and ERK 1/2 phosphorylation in the hippocampus of klotho mutant mice. Consistently, AG490, a JAK2/STAT3 inhibitor, dicyclomine, an M1 mAChR antagonist, or U0126, an ERK 1/2 inhibitor, significantly counteracted theanine-induced attenuation of memory impairment induced by klotho gene depletion in mice. Our study suggests that theanine attenuates memory impairments in a genetic aging model via up-regulation of JAK2/STAT3, M1 mAChR, and ERK signaling.
UR - http://www.scopus.com/inward/record.url?scp=85060205314&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060205314&partnerID=8YFLogxK
U2 - 10.1039/c8fo01577e
DO - 10.1039/c8fo01577e
M3 - Article
C2 - 30574980
AN - SCOPUS:85060205314
VL - 10
SP - 325
EP - 332
JO - Food and Function
JF - Food and Function
SN - 2042-6496
IS - 1
ER -