TY - JOUR
T1 - Theanine attenuates memory impairments induced by
T2 - Klotho gene depletion in mice
AU - Nguyen, Bao Trong
AU - Sharma, Naveen
AU - Shin, Eun Joo
AU - Jeong, Ji Hoon
AU - Lee, Sung Hoon
AU - Jang, Choon Gon
AU - Nah, Seung Yeol
AU - Nabeshima, Toshitaka
AU - Yoneda, Yukio
AU - Kim, Hyoung Chun
N1 - Funding Information:
This study was supported by the Basic Science Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT, & Future Planning (#NRF-2013R1A1A2060894 and #NRF-2016R1A1A1A050-055201), Republic of Korea. Bao Trong Nguyen and Naveen Sharma were supported by the BK21 PLUS program. We would like to express our thanks to Taiyo Kagaku Co., Ltd for the generous donation of theanine.
Publisher Copyright:
© 2019 The Royal Society of Chemistry.
PY - 2019/1
Y1 - 2019/1
N2 - Theanine (γ-glutamylethylamide), an amino acid in tea, is a putative neuroprotective and antioxidant compound capable of improving lifespan and cognitive function. Because we previously reported cognitive dysfunction in klotho mutant mice via down-regulation of janus kinase 2 (JAK2) and signal transducer and activator of transcription3 (STAT3), M1 muscarinic cholinergic receptor (M1 mAChR), and ERK signaling, we, therefore, investigated whether self-administration of theanine affects memory dysfunction in response to klotho gene depletion in mice, and whether theanine modulates the JAK2/STAT3, M1 mAChR, and ERK signaling network. Theanine significantly attenuated memory impairments in klotho mutant mice. Moreover, theanine self-administration significantly attenuated inhibitions of JAK2/STAT3 phosphorylation, M1 mAChR expression, and ERK 1/2 phosphorylation in the hippocampus of klotho mutant mice. Consistently, AG490, a JAK2/STAT3 inhibitor, dicyclomine, an M1 mAChR antagonist, or U0126, an ERK 1/2 inhibitor, significantly counteracted theanine-induced attenuation of memory impairment induced by klotho gene depletion in mice. Our study suggests that theanine attenuates memory impairments in a genetic aging model via up-regulation of JAK2/STAT3, M1 mAChR, and ERK signaling.
AB - Theanine (γ-glutamylethylamide), an amino acid in tea, is a putative neuroprotective and antioxidant compound capable of improving lifespan and cognitive function. Because we previously reported cognitive dysfunction in klotho mutant mice via down-regulation of janus kinase 2 (JAK2) and signal transducer and activator of transcription3 (STAT3), M1 muscarinic cholinergic receptor (M1 mAChR), and ERK signaling, we, therefore, investigated whether self-administration of theanine affects memory dysfunction in response to klotho gene depletion in mice, and whether theanine modulates the JAK2/STAT3, M1 mAChR, and ERK signaling network. Theanine significantly attenuated memory impairments in klotho mutant mice. Moreover, theanine self-administration significantly attenuated inhibitions of JAK2/STAT3 phosphorylation, M1 mAChR expression, and ERK 1/2 phosphorylation in the hippocampus of klotho mutant mice. Consistently, AG490, a JAK2/STAT3 inhibitor, dicyclomine, an M1 mAChR antagonist, or U0126, an ERK 1/2 inhibitor, significantly counteracted theanine-induced attenuation of memory impairment induced by klotho gene depletion in mice. Our study suggests that theanine attenuates memory impairments in a genetic aging model via up-regulation of JAK2/STAT3, M1 mAChR, and ERK signaling.
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U2 - 10.1039/c8fo01577e
DO - 10.1039/c8fo01577e
M3 - Article
C2 - 30574980
AN - SCOPUS:85060205314
SN - 2042-6496
VL - 10
SP - 325
EP - 332
JO - Food and Function
JF - Food and Function
IS - 1
ER -