Therapeutic effect of bifemeeane on unieateral cerebral ischemia in gerbils

Ryuji Hata, Masayasu Matsumoto, Kazuo Kitagawa, Masafumi Tagaya, Tomohiro Matsuyama, Minoru Sugita, Takenohu Kamada

Research output: Contribution to journalArticle

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Abstract

To examine the therapeutic effect of 4-(o-benzylphenoxy)-N-methylhutylamine hydrochloride (bifemelane) on neuronal ischemia-reperfusion injury, we evaluated the neurological score in gerbils subjected to unilateral common carotid occlusion. Using the neurological score method, we selected animals which showed stroke symptoms without seizures, because seizure activity can modify the neurological outcome. Just after the clip removal following 30 min of ischemia, 0.2 ml of either vehicle (0.9% saline, n = 10) or bifemelane (25 mg/kg, n = 10) was administered intraperitoneally. Then, the neurological score and the maximum inclination at which the gerbil could maintain its equilibrium were recorded at 5, 10, and 30 min, and 1, 2, 3, 6, and 24 hr after the insult. Animals were then decapitated and their brains were processed for immunohistochemical investigation. Treatment with bifemelane after reperfusion significantly improved the neurological score and motor function. Immunohistochemistry using an antibody for microtubule-associated protein 2 clearly demonstrated preservation of staining especially in the caudate-putamen in the animals treated with bifemelane. The present findings suggest a beneficial effect of bifemelane on neuronal ischemia-reperfusion injury, which may be common after cerebral ischemia.

Original languageEnglish
Pages (from-to)379-386
Number of pages8
JournalLife Sciences
Volume57
Issue number4
DOIs
Publication statusPublished - 16-06-1995

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Gerbillinae
Therapeutic Uses
Brain Ischemia
Animals
Reperfusion Injury
Seizures
Microtubule-Associated Proteins
Putamen
Surgical Instruments
Reperfusion
Brain
Ischemia
Immunohistochemistry
Stroke
bifemelane
Staining and Labeling
Antibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Hata, R., Matsumoto, M., Kitagawa, K., Tagaya, M., Matsuyama, T., Sugita, M., & Kamada, T. (1995). Therapeutic effect of bifemeeane on unieateral cerebral ischemia in gerbils. Life Sciences, 57(4), 379-386. https://doi.org/10.1016/0024-3205(95)00297-J
Hata, Ryuji ; Matsumoto, Masayasu ; Kitagawa, Kazuo ; Tagaya, Masafumi ; Matsuyama, Tomohiro ; Sugita, Minoru ; Kamada, Takenohu. / Therapeutic effect of bifemeeane on unieateral cerebral ischemia in gerbils. In: Life Sciences. 1995 ; Vol. 57, No. 4. pp. 379-386.
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Hata, R, Matsumoto, M, Kitagawa, K, Tagaya, M, Matsuyama, T, Sugita, M & Kamada, T 1995, 'Therapeutic effect of bifemeeane on unieateral cerebral ischemia in gerbils', Life Sciences, vol. 57, no. 4, pp. 379-386. https://doi.org/10.1016/0024-3205(95)00297-J

Therapeutic effect of bifemeeane on unieateral cerebral ischemia in gerbils. / Hata, Ryuji; Matsumoto, Masayasu; Kitagawa, Kazuo; Tagaya, Masafumi; Matsuyama, Tomohiro; Sugita, Minoru; Kamada, Takenohu.

In: Life Sciences, Vol. 57, No. 4, 16.06.1995, p. 379-386.

Research output: Contribution to journalArticle

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Hata R, Matsumoto M, Kitagawa K, Tagaya M, Matsuyama T, Sugita M et al. Therapeutic effect of bifemeeane on unieateral cerebral ischemia in gerbils. Life Sciences. 1995 Jun 16;57(4):379-386. https://doi.org/10.1016/0024-3205(95)00297-J