TY - JOUR
T1 - Third-generation cephalosporin-resistant non-typhoidal salmonella isolated from human feces in Japan
AU - Saito, Satomi
AU - Koori, Yoshio
AU - Ohsaki, Yusuke
AU - Osaka, Shunsuke
AU - Oana, Kozue
AU - Nagano, Yukiko
AU - Arakawa, Yoshichika
AU - Nagano, Noriyuki
N1 - Publisher Copyright:
© 2017, National Institute of Health. All rights reserved.
PY - 2017
Y1 - 2017
N2 - β-lactamase genes were detected and characterized from 10 non-typhoidal Salmonella (NTS) clinical isolates resistant to third-generation cephalosporins collected between 2012 and 2014 in Japan. Five strains showed cefotaxime minimum inhibitory concentration (MIC) ≥ 64 μg/ml and positive clavulanic acid inhibition results. The blaCTX-M-2 was detected in 3 strains (serotypes Stanley and Muenchen), whereas blaTEM-52 (serotype Manhattan) and blaSHV-12 (serotype Infantis) were each found in 1 strain. blaCMY-2 was detected in the remaining 5 strains (serotypes Infantis, Rissen, Newport, and Saintpaul) with cefotaxime MICs of 4–32 μg/ml and positive cloxacillin- and 3-aminophenylboronic ac-id-based inhibition tests. ISEcp1 was located upstream of the blaCMY-2 in 4 strains and of the blaCTX-M-2 in 1 strain. Incompatibility (Inc)A/C, IncP, and IncI1 plasmids were present in the strains harboring blaCMY-2, which were detected predominantly in this study. Acquisition of resistance to third-generation cephalosporins by invasive NTS may limit therapeutic options for severe systemic infections and causing serious public health problems. Though such resistant clinical isolates are still rare in Salmonella species in Japan, our findings reveal the presence of cephem-resistant NTS in food handlers, thus emphasizing the necessity of more systematic nationwide investigations.
AB - β-lactamase genes were detected and characterized from 10 non-typhoidal Salmonella (NTS) clinical isolates resistant to third-generation cephalosporins collected between 2012 and 2014 in Japan. Five strains showed cefotaxime minimum inhibitory concentration (MIC) ≥ 64 μg/ml and positive clavulanic acid inhibition results. The blaCTX-M-2 was detected in 3 strains (serotypes Stanley and Muenchen), whereas blaTEM-52 (serotype Manhattan) and blaSHV-12 (serotype Infantis) were each found in 1 strain. blaCMY-2 was detected in the remaining 5 strains (serotypes Infantis, Rissen, Newport, and Saintpaul) with cefotaxime MICs of 4–32 μg/ml and positive cloxacillin- and 3-aminophenylboronic ac-id-based inhibition tests. ISEcp1 was located upstream of the blaCMY-2 in 4 strains and of the blaCTX-M-2 in 1 strain. Incompatibility (Inc)A/C, IncP, and IncI1 plasmids were present in the strains harboring blaCMY-2, which were detected predominantly in this study. Acquisition of resistance to third-generation cephalosporins by invasive NTS may limit therapeutic options for severe systemic infections and causing serious public health problems. Though such resistant clinical isolates are still rare in Salmonella species in Japan, our findings reveal the presence of cephem-resistant NTS in food handlers, thus emphasizing the necessity of more systematic nationwide investigations.
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U2 - 10.7883/yoken.JJID.2016.166
DO - 10.7883/yoken.JJID.2016.166
M3 - Article
C2 - 27580578
AN - SCOPUS:85019959205
SN - 1344-6304
VL - 70
SP - 301
EP - 304
JO - Japanese journal of infectious diseases
JF - Japanese journal of infectious diseases
IS - 3
ER -
