TY - JOUR
T1 - Three different GB virus C/hepatitis G virus genotypes. Phylogenetic analysis and a genotyping assay based on restriction fragment length polymorphism
AU - Mukaide, Motokazu
AU - Mizokami, Masashi
AU - Orito, Etsuro
AU - Ohba, Ken ichi
AU - Nakano, Tatsunori
AU - Ueda, Ryuzo
AU - Hikiji, Kazumasa
AU - Iino, Shiro
AU - Shapiro, Sarah
AU - Lahat, Nitza
AU - Park, Young Min
AU - Kim, Boo Sung
AU - Oyunsuren, Tsendsuren
AU - Rezieg, Mohammed
AU - Al-Ahdal, Mohammed N.
AU - Lau, Johnson Y.N.
N1 - Funding Information:
Authors would like to thank Prof. M. Hayami, Institute for Virus Research, Kyoto University, for collecting the Cameroon samples. M.M. and S.I. were supported by grants in part by a Grant-in-Aid for Scientific Research (07041169) from the Japanese Ministry of Education, Science and Culture, from the Japanese Ministry of Health and Welfare, Health Science Research Grants (Non-A, non-B Hepatitis Research Grants), and by the Viral Hepatitis Research Foundation of Japan. J.Y.N. Lau was supported by a P.H.S. Grant AI41219.
PY - 1997/4/21
Y1 - 1997/4/21
N2 - The 5'-untranslated region (5'-UTR) sequences of 33 GB virus C/hepatitis G virus (GBV-C/HGV) obtained from different geographic areas were determined through reverse transcription polymerase chain reaction and dideoxy chain termination sequencing, the alignment of sequences, the estimation of the number of nucleotide substitution per site, and construction of phylogenetic trees. The 5'-UTR of GBV-HGV was found to be heterogeneous, with 70.9-99.5% homology. Three distinct phylogenetic branches mere observed consistently in all phylogenetic trees, GBV-C is the prototype for one, HGV for another, and there is a new branch which consisted of GBV-C/HGV isolates from Asia. Genotype-specific restriction sites for the restriction enzymes, ScrFI and BsmFI, were identified, and a simple restriction fragment polymorphism analysis was developed for genotyping. These data provide evidence that GBV-C/HGV consists of three different genotypes. Our simple genotyping assay will also provide a tool for epidemiological studies of GBV-C/HGV infection.
AB - The 5'-untranslated region (5'-UTR) sequences of 33 GB virus C/hepatitis G virus (GBV-C/HGV) obtained from different geographic areas were determined through reverse transcription polymerase chain reaction and dideoxy chain termination sequencing, the alignment of sequences, the estimation of the number of nucleotide substitution per site, and construction of phylogenetic trees. The 5'-UTR of GBV-HGV was found to be heterogeneous, with 70.9-99.5% homology. Three distinct phylogenetic branches mere observed consistently in all phylogenetic trees, GBV-C is the prototype for one, HGV for another, and there is a new branch which consisted of GBV-C/HGV isolates from Asia. Genotype-specific restriction sites for the restriction enzymes, ScrFI and BsmFI, were identified, and a simple restriction fragment polymorphism analysis was developed for genotyping. These data provide evidence that GBV-C/HGV consists of three different genotypes. Our simple genotyping assay will also provide a tool for epidemiological studies of GBV-C/HGV infection.
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U2 - 10.1016/S0014-5793(97)00136-1
DO - 10.1016/S0014-5793(97)00136-1
M3 - Article
C2 - 9141480
AN - SCOPUS:0031582211
SN - 0014-5793
VL - 407
SP - 51
EP - 58
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -