Three different GB virus C/hepatitis G virus genotypes. Phylogenetic analysis and a genotyping assay based on restriction fragment length polymorphism

Motokazu Mukaide, Masashi Mizokami, Etsuro Orito, Ken ichi Ohba, Tatsunori Nakano, Ryuzo Ueda, Kazumasa Hikiji, Shiro Iino, Sarah Shapiro, Nitza Lahat, Young Min Park, Boo Sung Kim, Tsendsuren Oyunsuren, Mohammed Rezieg, Mohammed N. Al-Ahdal, Johnson Y.N. Lau

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The 5'-untranslated region (5'-UTR) sequences of 33 GB virus C/hepatitis G virus (GBV-C/HGV) obtained from different geographic areas were determined through reverse transcription polymerase chain reaction and dideoxy chain termination sequencing, the alignment of sequences, the estimation of the number of nucleotide substitution per site, and construction of phylogenetic trees. The 5'-UTR of GBV-HGV was found to be heterogeneous, with 70.9-99.5% homology. Three distinct phylogenetic branches mere observed consistently in all phylogenetic trees, GBV-C is the prototype for one, HGV for another, and there is a new branch which consisted of GBV-C/HGV isolates from Asia. Genotype-specific restriction sites for the restriction enzymes, ScrFI and BsmFI, were identified, and a simple restriction fragment polymorphism analysis was developed for genotyping. These data provide evidence that GBV-C/HGV consists of three different genotypes. Our simple genotyping assay will also provide a tool for epidemiological studies of GBV-C/HGV infection.

Original languageEnglish
Pages (from-to)51-58
Number of pages8
JournalFEBS Letters
Volume407
Issue number1
DOIs
Publication statusPublished - 21-04-1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Three different GB virus C/hepatitis G virus genotypes. Phylogenetic analysis and a genotyping assay based on restriction fragment length polymorphism'. Together they form a unique fingerprint.

  • Cite this

    Mukaide, M., Mizokami, M., Orito, E., Ohba, K. I., Nakano, T., Ueda, R., Hikiji, K., Iino, S., Shapiro, S., Lahat, N., Park, Y. M., Kim, B. S., Oyunsuren, T., Rezieg, M., Al-Ahdal, M. N., & Lau, J. Y. N. (1997). Three different GB virus C/hepatitis G virus genotypes. Phylogenetic analysis and a genotyping assay based on restriction fragment length polymorphism. FEBS Letters, 407(1), 51-58. https://doi.org/10.1016/S0014-5793(97)00136-1