TY - JOUR
T1 - Three-Dimensional Localization of an Individual Fluorescent Molecule with Angstrom Precision
AU - Furubayashi, Taku
AU - Motohashi, Kazuya
AU - Wakao, Keisuke
AU - Matsuda, Tsuyoshi
AU - Kii, Isao
AU - Hosoya, Takamitsu
AU - Hayashi, Nobuhiro
AU - Sadaie, Mahito
AU - Ishikawa, Fuyuki
AU - Matsushita, Michio
AU - Fujiyoshi, Satoru
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/7/5
Y1 - 2017/7/5
N2 - Among imaging techniques, fluorescence microscopy is a unique method to noninvasively image individual molecules in whole cells. If the three-dimensional spatial precision is improved to the angstrom level, various molecular arrangements in the cell can be visualized on an individual basis. We have developed a cryogenic reflecting microscope with a numerical aperture of 0.99 and an imaging stability of 0.05 nm in standard deviation at a temperature of 1.8 K. The key optics to realize the cryogenic performances is the reflecting objective developed by our laboratory. With this cryogenic microscope, an individual fluorescent molecule (ATTO647N) at 1.8 K was localized with standard errors of 0.53 nm (x), 0.31 nm (y), and 0.90 nm (z) when 106 fluorescence photons from the molecule were accumulated in 5 min.
AB - Among imaging techniques, fluorescence microscopy is a unique method to noninvasively image individual molecules in whole cells. If the three-dimensional spatial precision is improved to the angstrom level, various molecular arrangements in the cell can be visualized on an individual basis. We have developed a cryogenic reflecting microscope with a numerical aperture of 0.99 and an imaging stability of 0.05 nm in standard deviation at a temperature of 1.8 K. The key optics to realize the cryogenic performances is the reflecting objective developed by our laboratory. With this cryogenic microscope, an individual fluorescent molecule (ATTO647N) at 1.8 K was localized with standard errors of 0.53 nm (x), 0.31 nm (y), and 0.90 nm (z) when 106 fluorescence photons from the molecule were accumulated in 5 min.
UR - https://www.scopus.com/pages/publications/85022091442
UR - https://www.scopus.com/pages/publications/85022091442#tab=citedBy
U2 - 10.1021/jacs.7b03899
DO - 10.1021/jacs.7b03899
M3 - Article
C2 - 28644014
AN - SCOPUS:85022091442
SN - 0002-7863
VL - 139
SP - 8990
EP - 8994
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 26
ER -